Bhujwalla Z M, Tozer G M, Field S B, Proctor E, Busza A, Williams S R
MRC Cyclotron Unit, Hammersmith Hospital, London, UK.
NMR Biomed. 1990 Aug;3(4):178-83. doi: 10.1002/nbm.1940030406.
Blood flow and phosphorus metabolites have been measured simultaneously in the murine RIF-1 tumour in vivo. Blood flow was measured using the H2 washout technique and 31P NMR spectroscopy was used to measure high energy phosphates, inorganic phosphate and intracellular pH within the tumour. Following NMR and flow measurements, hydralazine was administered and the measurements repeated. There was always a decrease in blood flow, and a fall in nucleoside triphosphate detected by NMR, after administration of hydralazine. At blood flows in excess of 15 mL 100 g-1 min-1, the nucleoside triphosphate/inorganic phosphate ratio was 1.0 or greater, whereas at flows in the range of 5-12 mL 100 g-1 min-1 the ratio fell to below 0.5, and at flows below 5 mL 100 g-1 min-1 there was no detectable resonance from nucleoside triphosphate.
已在小鼠RIF-1肿瘤体内同时测量了血流和磷代谢物。使用氢气洗脱技术测量血流，并使用31P核磁共振波谱法测量肿瘤内的高能磷酸盐、无机磷酸盐和细胞内pH值。在进行核磁共振和血流测量后，给予肼苯哒嗪并重复测量。给予肼苯哒嗪后，血流总是减少，核磁共振检测到的核苷三磷酸也会下降。当血流超过15 mL 100 g-1 min-1时，核苷三磷酸/无机磷酸盐比率为1.0或更高，而当血流在5-12 mL 100 g-1 min-1范围内时，该比率降至0.5以下，当血流低于5 mL 100 g-1 min-1时，未检测到核苷三磷酸的共振信号。
