Field S B, Needham S, Burney I A, Maxwell R J, Coggle J E, Griffiths J R
MRC Cyclotron Unit, Hammersmith Hospital, London, UK.
Br J Cancer. 1991 May;63(5):723-6. doi: 10.1038/bjc.1991.163.
The vast majority of studies on tumour vasculature are performed on transplanted tumours in rodents. However, it is known that there may be differences between primary and transplanted lesions. The purpose of this study is to test whether a specific vascular response is similar in primary tumours and in transplanted tumours derived from them. The technique used was to give an intraperitoneal injection of 5 mg kg-1 hydralazine, which is known to result in hypoxia in transplanted tumours. Changes in perfusion were indicated by changes in metabolism, monitored using 31P Magnetic Resonance Spectroscopy. The primary tumours were induced by local irradiation many months previously and only 4/11 (36%) of these responded to hydralazine. One of the non responders was subsequently transplanted into isogeneic mice to produce a tumour line which was histologically very similar to the primary. Of these 16/17 (94%) responded. The difference is statistically significant (P = 0.001). The reasons for this difference are not known. A number of possibilities are discussed and in the authors' opinion, the most likely cause is that it results from an artefact of transplantation.
绝大多数关于肿瘤血管系统的研究是在啮齿动物的移植肿瘤上进行的。然而，众所周知，原发性病变和移植性病变之间可能存在差异。本研究的目的是测试原发性肿瘤和由其衍生的移植肿瘤中特定的血管反应是否相似。所采用的技术是腹腔注射5 mg kg-1的肼屈嗪，已知该药物会导致移植肿瘤缺氧。灌注变化通过代谢变化来指示，使用31P磁共振波谱进行监测。原发性肿瘤是在数月前通过局部照射诱导产生的，其中只有4/11（36%）对肼屈嗪有反应。其中一个无反应者随后被移植到同基因小鼠中，产生了一个组织学上与原发性肿瘤非常相似的肿瘤系。在这些肿瘤中，16/17（94%）有反应。这种差异具有统计学意义（P = 0.001）。造成这种差异的原因尚不清楚。文中讨论了多种可能性，作者认为，最可能的原因是它源于移植的假象。
