Corn Host Plant Resistance Research Unit, U.S. Department of Agriculture-Agricultural Research Service, MS 39762, USA.
Toxins (Basel). 2011 Jul;3(7):754-65. doi: 10.3390/toxins3070754. Epub 2011 Jun 24.
A public candidate gene testing pipeline for resistance to aflatoxin accumulation or Aspergillus flavus infection in maize is presented here. The pipeline consists of steps for identifying, testing, and verifying the association of selected maize gene sequences with resistance under field conditions. Resources include a database of genetic and protein sequences associated with the reduction in aflatoxin contamination from previous studies; eight diverse inbred maize lines for polymorphism identification within any maize gene sequence; four Quantitative Trait Loci (QTL) mapping populations and one association mapping panel, all phenotyped for aflatoxin accumulation resistance and associated phenotypes; and capacity for Insertion/Deletion (InDel) and SNP genotyping in the population(s) for mapping. To date, ten genes have been identified as possible candidate genes and put through the candidate gene testing pipeline, and results are presented here to demonstrate the utility of the pipeline.
本文提出了一个用于玉米抗黄曲霉毒素积累或黄曲霉感染的公共候选基因检测方案。该方案包括鉴定、测试和验证所选玉米基因序列与田间条件下抗性关联的步骤。资源包括一个与减少先前研究中黄曲霉污染相关的遗传和蛋白质序列数据库；用于在任何玉米基因序列内鉴定多态性的八个不同的自交系；四个数量性状位点（QTL）作图群体和一个关联作图面板，均对黄曲霉毒素积累抗性和相关表型进行表型分析；以及在群体中进行插入/缺失（InDel）和 SNP 基因分型的能力，用于作图。迄今为止，已经确定了十个可能的候选基因，并通过候选基因检测方案进行了测试，这里呈现的结果证明了该方案的实用性。
