Winkle S A, Sheardy R D
Department of Chemistry, Florida International University, Miami 33199.
Biochemistry. 1990 Jul 10;29(27):6514-21. doi: 10.1021/bi00479a025.
We have previously shown that a short 16 base pair DNA oligomer can accommodate a B-Z conformational junction [Sheardy, R. D., & Winkle, S. A. (1989) Biochemistry 28, 720-725]. Results from 1H NMR studies indicated that only three base pairs were involved in the junction and that one of these base pairs was highly distorted. Being interested in the nature of this distortion, we constructed DNA oligomers which have the potential to contain multiple B-Z junctions for polyacrylamide electrophoretic studies. We report that the mobilities displayed by these molecules through acrylamide gels in the absence and presence of cobalt suggest that these molecules run shorter than they actually are. This anomalous migration may be due to structural/dynamic properties of the DNA helix manifested by the periodic distortions of the potential B-Z junctions.
我们之前已经表明，一个16个碱基对的短DNA寡聚物能够容纳一个B-Z构象连接点[谢迪，R.D.，&温克尔，S.A.（1989年）《生物化学》28卷，720 - 725页]。1H核磁共振研究结果表明，连接点中仅涉及三个碱基对，并且其中一个碱基对高度扭曲。出于对这种扭曲本质的兴趣，我们构建了有潜力包含多个B-Z连接点的DNA寡聚物用于聚丙烯酰胺电泳研究。我们报告称，这些分子在不存在和存在钴的情况下通过丙烯酰胺凝胶显示出的迁移率表明，这些分子的迁移长度比它们实际长度短。这种异常迁移可能是由于潜在B-Z连接点的周期性扭曲所表现出的DNA螺旋的结构/动态特性。
