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一种在高盐条件下含有B-Z连接的合成DNA寡聚物的初步光谱表征。

Preliminary spectroscopic characterization of a synthetic DNA oligomer containing a B-Z junction at high salt.

作者信息

Sheardy R D

机构信息

Department of Chemistry, Pennsylvania State University, Hazleton 18201.

出版信息

Nucleic Acids Res. 1988 Feb 11;16(3):1153-67. doi: 10.1093/nar/16.3.1153.

DOI:10.1093/nar/16.3.1153
PMID:3344203
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC334744/
Abstract

It is well known that the local conformation of a segment of DNA is dependent upon both the sequence of the segment and the conditions under which the DNA is prepared. In extreme cases, the DNA may contain regions of both right and left-handed conformations, mandating the existence of a conformational junction between the two. These B-Z junctions have been observed in plasmids but, to date, no model systems have been characterized to determine the molecular nature of these junctions. Preliminary CD, UV, and NMR studies on such a model are presented here. A 16 base pair oligonucleotide, containing a potential B-Z junction, has been synthesized and characterized by the above techniques. The results suggest that this molecule contains both right and left-handed conformations under condition of high salt, and thus a B-Z junction.

摘要

众所周知，一段DNA的局部构象取决于该片段的序列以及制备DNA时的条件。在极端情况下，DNA可能同时包含右手和左手构象区域，这就要求两者之间存在一个构象连接点。这些B-Z连接点已在质粒中被观察到，但迄今为止，尚未有用于确定这些连接点分子性质的模型系统得到表征。本文展示了对此类模型的初步圆二色光谱（CD）、紫外（UV）和核磁共振（NMR）研究。一个含有潜在B-Z连接点的16碱基对寡核苷酸已被合成，并通过上述技术进行了表征。结果表明，该分子在高盐条件下同时包含右手和左手构象，因此存在一个B-Z连接点。

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本文引用的文献

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Nucleic Acids Res. 1983 Jul 11;11(13):4453-66. doi: 10.1093/nar/11.13.4453.
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B to Z transition of double-stranded poly[deoxyguanylyl(3'-5')-5-methyldeoxycytidine] in solution by phosphorus-31 and carbon-13 nuclear magnetic resonance spectroscopy.通过磷-31和碳-13核磁共振光谱法研究双链聚[脱氧鸟苷酰（3'-5'）-5-甲基脱氧胞苷]在溶液中的B到Z转变
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