Nguyen T D, Le Q D, Huynh V V, Nguyen S T, Nguyen T V, Vu-Khac H
Department of Parasitology, Central Vietnam Veterinary Institute, Nha Trang, Vietnam.
J Helminthol. 2012 Dec;86(4):426-9. doi: 10.1017/S0022149X11000629. Epub 2011 Nov 10.
The aims of this study were to investigate the prevalence of Moniezia spp. in domestic ruminants in central Vietnam and to develop a polymerase chain reaction (PCR) technique to distinguish M. expansa from M. benedeni. Among 2040 examined domestic animals (540 cattle, 800 goats, 700 sheep) Moniezia was recovered from 5.4% of cattle, 16.4% of sheep and 20.6% of goats. A set of primers for PCR was designed to classify M. expansa and M. benedeni based on the amplification of DNA corresponding to the internal transcribed spacer of 5.8S rRNA. The 457 specimens (75 from cattle, 162 from goats, 150 from sheep, 30 from horses, 30 from chickens and 10 from dogs) were subjected to PCR for classification of Moniezia spp. PCR products with the expected sizes were amplified from bovine, ovine and caprine specimens. No specific PCR products were found for specimens from horses, chickens and dogs. Of the 75 specimens from cattle, nine were classified as M. expansa and 66 were M. benedeni. Among 162 caprine specimens, 138 were M. expansa and 24 were M. benedeni. The distribution of M. expansa and M. benedeni in 150 ovine specimens was 132 and 18, respectively. These results show that M. expansa is dominant in goats and sheep, whereas M. benedeni is more common in cattle; PCR can be used for classification of these two species.
本研究的目的是调查越南中部地区家畜体内扩展莫尼茨绦虫（Moniezia expansa）和贝氏莫尼茨绦虫（Moniezia benedeni）的感染率，并开发一种聚合酶链反应（PCR）技术以区分这两种绦虫。在2040头被检查的家畜（540头牛、800只山羊、700只绵羊）中，莫尼茨绦虫的检出率分别为：牛5.4%、绵羊16.4%、山羊20.6%。设计了一组用于PCR的引物，根据对应于5.8S rRNA内部转录间隔区的DNA扩增来区分扩展莫尼茨绦虫和贝氏莫尼茨绦虫。对457份样本（75份来自牛、162份来自山羊、150份来自绵羊、30份来自马、30份来自鸡、10份来自狗）进行PCR以对莫尼茨绦虫属进行分类。从牛、羊和山羊样本中扩增出了预期大小的PCR产物。在马、鸡和狗的样本中未发现特异性PCR产物。在75份牛样本中，9份被分类为扩展莫尼茨绦虫，66份为贝氏莫尼茨绦虫。在162份山羊样本中，138份为扩展莫尼茨绦虫，24份为贝氏莫尼茨绦虫。在150份绵羊样本中，扩展莫尼茨绦虫和贝氏莫尼茨绦虫的分布分别为132份和18份。这些结果表明，扩展莫尼茨绦虫在山羊和绵羊中占主导地位，而贝氏莫尼茨绦虫在牛中更常见；PCR可用于这两个物种的分类。
