Department of Obstetrics and Gynaecology, Konkuk University Medical Centre, Konkuk University School of Medicine, Seoul, Republic of Korea.
Eur J Obstet Gynecol Reprod Biol. 2012 Feb;160(2):137-41. doi: 10.1016/j.ejogrb.2011.10.007. Epub 2011 Nov 8.
To investigate the expression pattern of CXC chemokine ligand-12 (CXCL12) in the placentae of normal and pre-eclamptic women.
Twenty-five women with severe pre-eclampsia and 30 normotensive women, matched for gestational age, were enrolled in the study. Placental tissue from each woman was collected following delivery by caesarean section. Quantitative reverse transcription polymerase chain reaction, Western blot analysis and immunohistochemical staining were performed for mRNA expression, quantification and tissue localization of CXCL12 in each placenta.
CXCL12 expression was greater in pre-eclamptic placentae compared with normal placentae. CXCL12 was detected in most placental tissue cells by immunohistochemical staining. CXCL12 immunoreactivity was significantly greater in syncytiotrophoblasts of pre-eclamptic placentae compared with normal placentae. However, there was no significant difference in CXCL12 immunoreactivity in other tissues between the two groups.
CXCL12 expression is significantly greater in the placentae of pre-eclamptic women compared with normal women. This may represent part of a compensatory mechanism for pre-eclampsia.
研究 CXC 趋化因子配体-12（CXCL12）在正常妊娠妇女和子痫前期妇女胎盘组织中的表达模式。
本研究纳入了 25 例重度子痫前期妇女和 30 例年龄匹配的正常血压妇女。所有妇女均通过剖宫产分娩后收集胎盘组织。采用定量逆转录聚合酶链反应、Western blot 分析和免疫组织化学染色检测胎盘组织中 CXCL12 的 mRNA 表达、定量和组织定位。
与正常胎盘相比，子痫前期胎盘组织中 CXCL12 的表达更高。免疫组织化学染色显示 CXCL12 在大多数胎盘组织细胞中均有表达。与正常胎盘相比，子痫前期胎盘组织中的合体滋养层细胞中 CXCL12 的免疫反应性显著增强。然而，两组间其他组织中 CXCL12 的免疫反应性无显著差异。
与正常孕妇相比，子痫前期孕妇的胎盘组织中 CXCL12 的表达显著增加。这可能是子痫前期的一种代偿机制的一部分。
