通过 NMR 光谱学对前列腺相关基因（PAGE5）进行单残基分辨率的无规卷曲结构分析。

Characterization of intrinsically disordered prostate associated gene (PAGE5) at single residue resolution by NMR spectroscopy.

机构信息

Program in Structural Biology and Biophysics, Institute of Biotechnology, University of Helsinki, Helsinki, Finland.

出版信息

PLoS One. 2011;6(11):e26633. doi: 10.1371/journal.pone.0026633. Epub 2011 Nov 2.

BACKGROUND

The Cancer-Testis antigens (CTA) are proteins expressed in human germ line and certain cancer cells. CTAs form a large gene family, representing 10% of X-chromosomal genes. They have high potential for cancer-specific immunotherapy. However, their biological functions are currently unknown. Prostate associated genes (PAGE) are characterized as CTAs. PAGE5 is one of six proteins belonging to this protein family, also called CT16.

METHODOLOGY/PRINCIPAL FINDINGS: In this study we show, using bioinformatics, chromatographic and solution state NMR spectroscopic methods, that PAGE5 is an intrinsically disordered protein (IDP).

CONCLUSION/SIGNIFICANCE: The study stands out as the first time structural characterization of the PAGE family protein and introduces how solution state NMR spectroscopy can be effectively utilized for identification of molecular recognition regions (MoRF) in IDPs, known often as transiently populated secondary structures.

背景

癌症睾丸抗原（CTA）是在人类生殖系和某些癌细胞中表达的蛋白质。CTA 形成一个大的基因家族，占 X 染色体基因的 10%。它们具有用于癌症特异性免疫治疗的巨大潜力。然而，它们的生物学功能目前尚不清楚。前列腺相关基因（PAGE）是 CTA 的特征。PAGE5 是属于该蛋白质家族的六个蛋白质之一，也称为 CT16。

方法/主要发现：在这项研究中，我们使用生物信息学、色谱和溶液状态 NMR 光谱学方法表明，PAGE5 是一种固有无序的蛋白质（IDP）。

结论/意义：该研究首次对 PAGE 家族蛋白进行结构特征描述，并介绍了如何有效地利用溶液状态 NMR 光谱学来鉴定 IDP 中的分子识别区域（MoRF），这些区域通常被称为短暂存在的二级结构。