Research Center of Material Science, Beijing Institute of Technology, Beijing, China.
J Mater Sci Mater Med. 2012 Feb;23(2):315-24. doi: 10.1007/s10856-011-4476-3. Epub 2011 Nov 11.
A novel all-aqueous process is described to form three-dimensional porous silk fibroin (SF) scaffolds, which not only avoided the use of organic solvents or harsh chemicals, but also can form scaffolds with various sizes and in large quantities. The scaffolds show a rough surface on the pores and the pores are highly interconnected. The porosity of the scaffolds, which varied between a large range (67.6~99.3%), can be controlled by the SF concentrations and the salt/fibroin ratio. The results of measurements indicated that this novel process can improve and enforce the transformation in SF structure from a random coil to a β-sheet. Swelling studies showed that the scaffold has excellent properties of hydrophilicity. The cell culture experiments demonstrated that the scaffolds facilitated the human osteosarcoma cells attachment and proliferation in vitro.
本文描述了一种新颖的全水相工艺来制备三维多孔丝素（SF）支架，该方法不仅避免了有机溶剂或苛刻化学物质的使用，而且可以大量制备具有不同尺寸的支架。支架的孔呈现出粗糙的表面，并且孔高度互联。支架的孔隙率在很大范围内变化（67.6％至 99.3％），可以通过 SF 浓度和盐/丝素比来控制。测量结果表明，这种新方法可以改善和增强 SF 结构从无规卷曲到β-折叠的转变。溶胀研究表明，支架具有优异的亲水性。细胞培养实验表明，支架有利于人骨肉瘤细胞在体外的附着和增殖。
