Lv Qiang, Cao Chuanbao, Zhang Ying, Man Xilan, Zhu Hesun
Research Center of Material Science, Beijing Institute of Technology, Beijing 100081, Republic of China.
J Mater Sci Mater Med. 2004 Nov;15(11):1193-7. doi: 10.1007/s10856-004-5918-y.
Blending degummed fibroin (DF) or insoluble fibroin microspheres with concentrated fibroin solution, the insoluble films were obtained through drying the solution at 40-45 degrees C. The conformation of silk fibroin films was analyzed by infrared spectrum and X-ray diffractometry. The results demonstrated that beta-sheet conformation increased rapidly when the degummed silk or insoluble microspheres blended with fibroin, while the pure SF membrane was mainly composed of alpha/random coil conformation when the other conditions kept same. This suggested that fibroin microspheres and degummed fibroin could induce the formation of beta-sheet crystal and the insoluble films, without methanol after-treatment, could be obtained at approximately room temperature. Although the fibroin films blending with DF had many protuberances, the films containing fibroin microspheres had the smooth surface and could be used effectively in biotechnological materials and biomedical application.
将脱胶丝素蛋白（DF）或不溶性丝素蛋白微球与浓缩丝素蛋白溶液混合，通过在40 - 45摄氏度下干燥溶液获得不溶性薄膜。通过红外光谱和X射线衍射法分析丝素蛋白薄膜的构象。结果表明，当脱胶丝或不溶性微球与丝素蛋白混合时，β - 折叠构象迅速增加，而在其他条件相同的情况下，纯丝素蛋白膜主要由α/无规卷曲构象组成。这表明丝素蛋白微球和脱胶丝素蛋白可诱导β - 折叠晶体的形成，并且无需甲醇后处理即可在大约室温下获得不溶性薄膜。尽管与DF混合的丝素蛋白膜有许多突起，但含有丝素蛋白微球的薄膜表面光滑，可有效地用于生物技术材料和生物医学应用。
