Ayeh Kwadwo Owusu, Lee Yeonkyeong, Ambrose Mike J, Hvoslef-Eide Anne Kathrine
Department of Plant and Environmental Sciences, Norwegian University of Life Sciences, P,O, BOX 5003, 1432 Aas, Norway.
BMC Res Notes. 2011 Nov 11;4:489. doi: 10.1186/1756-0500-4-489.
The def mutant pea (Pisum sativum L) showed non-abscission of seeds from the funicule. Here we present data on seed development and growth pattern and their relationship in predicting this particular trait in wild type and mutant lines as well as the inheritance pattern of the def allele in F2 and F3 populations.
Pod length and seed fresh weight increase with fruit maturity and this may affect the abscission event in pea seeds. However, the seed position in either the distal and proximal ends of the pod did not show any difference. The growth factors of seed fresh weight (FW), width of funicles (WFN), seed width (SW) and seed height (SH) were highly correlated and their relationships were determined in both wild type and def mutant peas. The coefficient of determination R2 values for the relationship between WFN and FW, SW and SH and their various interactions were higher for the def dwarf type. Stepwise multiple regression analysis showed that variation of WFN was associated with SH and SW. Pearson's chi square analysis revealed that the inheritance and segregation of the Def locus in 3:1 ratio was significant in two F2 populations. Structural analysis of the F3 population was used to confirm the inheritance status of the Def locus in F2 heterozygote plants.
This study investigated the inheritance of the presence or absence of the Def allele, controlling the presence of an abscission zone (AZ) or an abscission-less zone (ALZ) forming in wild type and mutant lines respectively. The single major gene (Def) controlling this phenotype was monogenic and def mutants were characterized and controlled by the homozygous recessive def allele that showed no palisade layers in the hilum region of the seed coat.
def突变型豌豆（Pisum sativum L）表现出种子与珠柄不脱落的现象。在此，我们展示了关于种子发育、生长模式及其在预测野生型和突变系中这一特定性状的关系的数据，以及F2和F3群体中def等位基因的遗传模式。
荚果长度和种子鲜重随果实成熟而增加，这可能影响豌豆种子的脱落事件。然而，种子在荚果远端和近端的位置没有显示出任何差异。种子鲜重（FW）、珠柄宽度（WFN）、种子宽度（SW）和种子高度（SH）的生长因子高度相关，并在野生型和def突变型豌豆中确定了它们之间的关系。对于def矮化类型，WFN与FW、SW与SH及其各种相互作用之间关系的决定系数R2值更高。逐步多元回归分析表明，WFN的变化与SH和SW相关。Pearson卡方分析显示，Def位点在两个F2群体中以3:1的比例遗传和分离是显著的。F3群体的结构分析用于确认F2杂合子植物中Def位点的遗传状态。
本研究调查了Def等位基因存在与否的遗传情况，该等位基因分别控制野生型和突变系中脱落区（AZ）或无脱落区（ALZ）的形成。控制这一表型的单个主基因（Def）是单基因的，def突变体的特征是由纯合隐性def等位基因控制，该等位基因在种皮脐部区域没有栅栏层。
