Rice Genome Research Program, National Institute of Agrobiological Resources/Institue of Society for Techno-Innovation of Agriculture, Forestry, and Fisheries, 446-1 Ippaizuka, Kamiyokoba, Tsukuba, 305, Ibaraki, Japan.
Theor Appl Genet. 1996 Feb;92(2):145-50. doi: 10.1007/BF00223368.
We have constructed a high-resolution rice genetic map containing 1383 DNA markers covering 1575 cM on the 12 linkage groups of rice using 186 F2 progeny from a cross between a japonica variety, 'Nipponbare', and an indica variety, 'Kasalath'. Using this high-resolution molecular linkage map, we detected segregation distortion in a single wide cross of rice. The frequencies of genotypes for 1181 markers with more than 176 genotype data were plotted along this map to detect segregation distortion. Several types of distorted segregation were observed on 6 of the chromosomes. We could detect 11 major segregation distortions at ten positions on chromosomes 1, 3, 6, 8, 9, and 10. The strongest segregation distortion was at 107.2 cM on chromosome 3 and may be the gametophyte gene 2 (ga-2). The 'Kasalath' genotype at this position was transmitted to the progeny with about a 95% probability through the pollen gamete. At least 8 out of the 11 segregation distortions detected here are new. The use of the high-resolution molecular linkage map for improving our understanding of the genetic nature and cause of these segregation distortions is discussed.
我们利用‘Nipponbare’（粳稻品种）和‘Kasalath’（籼稻品种）杂交的 186 个 F2 后代，构建了一个包含 1383 个 DNA 标记、覆盖水稻 12 条连锁群 1575cM 的高分辨率水稻遗传图谱。利用这个高分辨率分子连锁图谱，我们检测到了一个水稻的宽杂交分离失真。在这条图谱上，我们对 1181 个标记的基因型进行了超过 176 个基因型数据的作图，以检测分离失真。在 6 条染色体上观察到了几种类型的偏分离。我们在染色体 1、3、6、8、9 和 10 上的 10 个位置检测到了 11 个主要的分离偏度。3 号染色体上最强的分离偏度发生在 107.2cM，可能是配子体基因 2（ga-2）。该位置的‘Kasalath’基因型通过花粉配子以约 95%的概率传递给后代。在这里检测到的 11 个分离偏度中，至少有 8 个是新的。利用高分辨率分子连锁图谱来提高我们对这些分离偏度遗传性质和原因的理解，是我们正在讨论的。
