Hebei Key Laboratory of Molecular and Cellular Biology, College of Life Sciences, Hebei Normal University, Shijiazhuang, Hebei 050016, China.
Plant Physiol Biochem. 2011 Dec;49(12):1384-91. doi: 10.1016/j.plaphy.2011.09.012. Epub 2011 Oct 1.
The DREB and CBF transcription factors play a critical role in plant development and abiotic stress responses and, therefore, represent attractive targets for a molecular plant breeding approach. In this study, the rice OsDREB2A gene was isolated and expressed under the control of a stress-inducible promoter (4ABRC) to improve the abiotic stress tolerance of japonica rice variety TNG67. T₂ and T₃ transgenic lines over-expressing OsDREB2A were found to have improved survival rates under severe drought and salt stress conditions relative to non-transgenic rice plants or rice plants transformed with the empty vector control. OsDREB2A expression was found to be markedly induced by drought and ABA treatment. The results indicate that the induced over-expression of OsDREB2A driven by the 4ABRC promoter in engineered rice plants may protect cells during stress.
DREB 和 CBF 转录因子在植物发育和非生物胁迫响应中起着关键作用，因此，它们是分子植物育种方法的有吸引力的目标。在这项研究中，从水稻中分离出 OsDREB2A 基因，并在胁迫诱导启动子（4ABRC）的控制下表达，以提高粳稻品种 TNG67 的非生物胁迫耐受性。与非转基因水稻或转空载体对照的水稻相比，过量表达 OsDREB2A 的 T₂和 T₃ 转基因株系在严重干旱和盐胁迫条件下的存活率有所提高。发现 OsDREB2A 的表达受干旱和 ABA 处理的显著诱导。结果表明，在工程水稻植物中，由 4ABRC 启动子驱动的 OsDREB2A 的诱导过表达可能在胁迫期间保护细胞。
