He Kai-liang, Li Yong-ping, Lv Yong-gang, Zhang Dan-jie, Wang Hui
Department of General Surgery, Hospital of Northwestern Polytechnical University, Xi'an, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2011 Nov;27(11):1220-2.
To study possible association between three single nucleotide polymorphisms (-597G/A, -572C/G and -174G/C) of interleukin-6 (IL-6) gene promoter and breast cancer.
Genomic DNA was isolated from the venous blood leukocytes from 176 unrelated patients with breast cancer and 200 healthy unrelated females (control group). Polymorphisms of -597G/A, -572C/G and -174G/C, were genotyped by PCR-restriction fragment length polymorphisms (PCR-RFLP). SPSS 11.5 software was employed for statistical analysis and the association of IL-6 polymorphisms with breast cancer was evaluated by x(2); test.
There was significant differences in both allele and genotype frequencies of -572C/G in case group compared with control group. The allele G of -572C/G was significantly higher in cancer patients than the controls(x(2);=15.438, P<0.01).
There is an association between -572C/G polymorphism and breast cancer risk. The females with G allele of -572C/G are susceptible to breast cancer compared with non-carrying females.
研究白细胞介素-6（IL-6）基因启动子的三个单核苷酸多态性（-597G/A、-572C/G和-174G/C）与乳腺癌之间可能存在的关联。
从176例无亲缘关系的乳腺癌患者及200名无亲缘关系的健康女性（对照组）的静脉血白细胞中提取基因组DNA。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术对-597G/A、-572C/G和-174G/C多态性进行基因分型。使用SPSS 11.5软件进行统计分析，并通过χ²检验评估IL-6基因多态性与乳腺癌的相关性。
病例组与对照组相比，-572C/G的等位基因频率和基因型频率均存在显著差异。-572C/G的等位基因G在癌症患者中显著高于对照组（χ²=15.438，P<0.01）。
-572C/G多态性与乳腺癌风险之间存在关联。与未携带该等位基因的女性相比，携带-572C/G等位基因G的女性易患乳腺癌。
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