Fu Hai-xia, Zhang Jia-ying, Li Geng-shan, Li Yan, Xu Jia-li, Zhao Zi-niu
Department of Cardiology, Henan Provincial People's Hospital, Zhengzhou, Henan, 450003 PR China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2006 Jun;23(3):245-9.
To observe the polymorphism and gene frequency of interleukin 6 (IL6) gene -572C/G in Chinese Han nationality population, that associating with susceptibility to myocardial infarction(MI) and impacting on the extent of coronary artery lesions; to analyze the function of IL6 gene -572C/G polymorphism.
With PCR-RFLP method, IL6 gene -572C/G polymorphism was genotyped to 232 MI patients and 260 healthy adults. The effect of IL6 gene -572C/G polymorphism was observed to the extent of coronary artery lesions and the ability of IL6 production from peripheral blood mononuclear cells (PBMC).
There was IL6 gene -572C/G polymorphism in Chinese Hans. -572CG+GG genotype and G allele were more frequent in patients than in controls (P< 0.01). The relative risk for G allele carrier to suffer from MI was 1.68 times of CC genotype individual (95%CI 1.17-2.41, P< 0.01). However, the distribution of IL6 gene -572C/G polymorphism was no significant difference among patients with single-vessel, two-vessel and three-vessel lesions (P> 0.05). After PBMC cultured for 24 hours, the IL6 concentration in supernatant was significantly higher in subjects with CG genotype than those with CC genotype (P< 0.05).
IL6 gene -572G allele may be a genetic susceptibility factor to MI attack of Chinese Hans population, and related to the high expression of IL6.
观察白细胞介素6（IL6）基因-572C/G在中国汉族人群中的多态性及基因频率，探讨其与心肌梗死（MI）易感性的关系以及对冠状动脉病变程度的影响；分析IL6基因-572C/G多态性的功能。
采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法，对232例MI患者和260例健康成年人进行IL6基因-572C/G多态性基因分型。观察IL6基因-572C/G多态性对冠状动脉病变程度及外周血单个核细胞（PBMC）产生IL6能力的影响。
中国汉族人群存在IL6基因-572C/G多态性。患者中-572CG+GG基因型和G等位基因频率高于对照组（P<0.01）。G等位基因携带者患MI的相对风险是CC基因型个体的1.68倍（95%CI 1.17-2.41，P<0.01）。然而，IL6基因-572C/G多态性在单支、双支和三支血管病变患者中的分布无显著差异（P>0.05）。PBMC培养24小时后，CG基因型受试者上清液中IL6浓度显著高于CC基因型受试者（P<0.05）。
IL6基因-572G等位基因可能是中国汉族人群MI发病的遗传易感因素，并与IL6的高表达有关。
