Department of Cardiology, First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
Chin Med J (Engl). 2012 Nov;125(22):3997-4002.
Increased levels of interleukin-6 (IL-6) and C-reactive protein (CRP) have been reported in patients with venous thromboembolisms (VTE). However, prospective studies did not confirm an association between IL-6, CRP and their polymorphism with the risk of VTE.
One hundred and forty patients (including 66 males and 74 females, mean age (55.55 ± 17.11) years) and one hundred and sixty controls (including 74 males and 86 females, mean age (56.58 ± 12.24) years) were involved. An enzyme linked immunosorbent assay (ELISA) method was used for detecting the serum levels of inflammatory factors IL-6 and CRP in both groups. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used for analyzing the distribution of polymorphisms at the -572C/G and -597G/A sites of the promoter of the IL-6 gene and at 1059G/C of the CRP gene.
Serum levels of IL-6 and CRP were significantly higher in the VTE group than in the control group (P < 0.05). The frequencies of -572C/G promoter polymorphisms CC, CG, and GG in the IL-6 gene were found to be 34%, 48%, and 18%, respectively, and the derived allele frequencies for the C and G alleles were 58% and 42%. There was a significant difference in the -572C/G promoter polymorphisms between the VTE group and control group (P < 0.05). For the -597G/A polymorphism, individuals all carried the GG and GA type; AA genotypes were not detected. The frequency of the GG, GC, and CC genotypes at the CRP1059G/C promoter was 87.57%, 7.86% and 3.57% in VTE group, while 86.25%, 10%, and 3.75% in control group, respectively. The frequency of G and C alleles at CRP 1059G/C was 91.43% and 8.57% in VTE group and 91.56% and 8.44% in the control group. The results showed that there was no statistically significant difference of 1059G/C genotype and mutation frequency of the allele between the VTE group and control group (P > 0.05). Multiple Logistic regression analysis showed CC homozygotes of the IL-6 -572G/C, body mass index (BMI), and CRP, IL-6, and high-density lipoprotein cholesterol (HDL-C) were independent risk factors for VTE (P < 0.05).
We found that VTE was associated with IL-6 and CRP levels, and there was an association of IL-6 and its promoter polymorphism at -572G/C with the risk of VTE. Thus far, a causal relationship between inflammation and VTE remains to be clarified and more prospective data are required.
已有研究报道静脉血栓栓塞症（VTE）患者的白细胞介素 6（IL-6）和 C 反应蛋白（CRP）水平升高。然而，前瞻性研究并未证实 IL-6、CRP 及其多态性与 VTE 风险之间存在关联。
纳入 140 例患者（包括 66 名男性和 74 名女性，平均年龄（55.55±17.11）岁）和 160 名对照者（包括 74 名男性和 86 名女性，平均年龄（56.58±12.24）岁）。采用酶联免疫吸附试验（ELISA）方法检测两组血清中炎症因子 IL-6 和 CRP 的水平。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析 IL-6 基因启动子-572C/G 和-597G/A 位点以及 CRP 基因 1059G/C 多态性的分布。
VTE 组血清 IL-6 和 CRP 水平明显高于对照组（P<0.05）。IL-6 基因-572C/G 启动子多态性 CC、CG 和 GG 的频率分别为 34%、48%和 18%，C 和 G 等位基因的衍生等位基因频率分别为 58%和 42%。VTE 组与对照组比较，-572C/G 启动子多态性差异有统计学意义（P<0.05）。-597G/A 多态性中，个体均携带 GG 和 GA 型；未检测到 AA 基因型。CRP1059G/C 启动子 GG、GC 和 CC 基因型在 VTE 组的频率分别为 87.57%、7.86%和 3.57%，在对照组的频率分别为 86.25%、10%和 3.75%。CRP 1059G/C 处 G 和 C 等位基因的频率在 VTE 组分别为 91.43%和 8.57%，在对照组分别为 91.56%和 8.44%。结果显示，VTE 组与对照组 1059G/C 基因型和等位基因突变频率无统计学差异（P>0.05）。多因素 Logistic 回归分析显示，IL-6-572G/C 纯合子、体重指数（BMI）、CRP、IL-6 和高密度脂蛋白胆固醇（HDL-C）是 VTE 的独立危险因素（P<0.05）。
我们发现 VTE 与 IL-6 和 CRP 水平有关，IL-6 及其启动子-572G/C 多态性与 VTE 的风险有关。到目前为止，炎症与 VTE 之间的因果关系仍需阐明，需要更多的前瞻性数据。
