Department of Biochemistry, Gulbarga University, Gulbarga 585106, Karnataka, India.
J Hazard Mater. 2012 Jan 15;199-200:58-63. doi: 10.1016/j.jhazmat.2011.10.053. Epub 2011 Nov 3.
Effective removal of dimethylformamide (DMF), the organic solvent found in industrial effluents of textile and pharma industries, was demonstrated by using free and immobilized cells of Ochrobactrum sp. DGVK1, a soil isolate capable of utilizing DMF as a sole source of carbon, nitrogen. The free cells have efficiently removed DMF from culture media and effluents, only when DMF concentration was less than 1% (v/v). Entrapment of cells either in alginate or in polyvinyl alcohol (PVA) failed to increase tolerance limits. However, the cells of Ochrobactrum sp. DGVK1 entrapped in PVA-alginate mixed matrix tolerated higher concentration of DMF (2.5%, v/v) and effectively removed DMF from industrial effluents. As determined through batch fermentation, these immobilized cells have retained viability and degradability for more than 20 cycles. A continuous packed bed reactor, generated by using PVA-alginate beads, efficiently removed DMF from industrial effluents, even in the presence of certain organic solvents frequently found in effluents along with DMF.
采用能够利用 DMF 作为唯一碳源和氮源的土壤分离株 Ochrobactrum sp. DGVK1 的游离细胞和固定化细胞，有效地去除了纺织和制药工业工业废水中的有机溶剂二甲基甲酰胺（DMF）。只有当 DMF 浓度低于 1%（v/v）时，游离细胞才能从培养基和废水中有效地去除 DMF。细胞在藻酸盐或聚乙烯醇（PVA）中的包埋都未能提高耐受限度。然而，包埋在 PVA-藻酸盐混合基质中的 Ochrobactrum sp. DGVK1 细胞能够耐受更高浓度的 DMF（2.5%，v/v），并有效地从工业废水中去除 DMF。通过分批发酵确定，这些固定化细胞在 20 多次循环中保持了活力和可降解性。使用 PVA-藻酸盐珠粒生成的连续填充床反应器能够有效地从工业废水中去除 DMF，即使在存在与 DMF 一起经常在废水中发现的某些有机溶剂的情况下也是如此。
