Institute of Phytopathology and Applied Zoology, Justus Liebig University of Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.
Dev Genes Evol. 2011 Dec;221(5-6):357-62. doi: 10.1007/s00427-011-0379-z. Epub 2011 Nov 12.
Heat shock protein 90 (HSP90) belongs to a family of conserved chaperons with multiple roles in stress adaptation and development, including spermatogenesis, oogenesis and embryogenesis in insects. In the red flour beetle, Tribolium castaneum, we found that HSP90 is transiently upregulated during larval development, in prepupae, in female pupae and in adults, suggesting multiple post-embryonic roles. We found that silencing HSP90 expression by RNA interference was lethal within 10 days at all developmental stages. Titration experiments revealed that larvae were more susceptible than pupae or beetles. Interestingly, HSP90 silencing in final instar larvae resulted in abnormal pupal phenotypes lacking compound eyes and exhibiting prepupal features, suggesting developmental arrest at the prepupal stage. Our results suggest that HSP90 functions can be expanded beyond the known ones in insect embryogenesis to include roles in post-embryonic development such as the regulation of compound eye development.
热休克蛋白 90（HSP90）属于一组保守伴侣蛋白家族，在应激适应和发育中具有多种作用，包括昆虫的精子发生、卵子发生和胚胎发生。在红粉甲虫（Tribolium castaneum）中，我们发现 HSP90 在幼虫发育过程中、预蛹期、雌性蛹期和成虫期都有短暂的上调，表明其具有多种胚胎后作用。我们发现通过 RNA 干扰沉默 HSP90 表达在所有发育阶段的 10 天内都具有致死性。滴定实验表明，幼虫比蛹或甲虫更易受影响。有趣的是，终龄幼虫中 HSP90 的沉默导致异常蛹表型，缺乏复眼并表现出预蛹特征，表明发育停滞在预蛹期。我们的结果表明，HSP90 的功能可以扩展到已知的昆虫胚胎发生之外，包括在胚胎后发育中发挥作用，例如调节复眼发育。
