Lehtinen P
Acta Chem Scand B. 1979;33(3):171-4. doi: 10.3891/acta.chem.scand.33b-0171.
Nuclei isolated from proliferating granulation tissue were incubated with 20 000 g supernatants from untreated and SiO2-treated subcellular particles of rat peritoneal macrophages in the presence of radioactive nucleic acid precursors. The supernatant from SiO2-treated subcellular particles increased the incorporation of [3H]CTP into nuclear RNA maximally by 26% at 5 min, and that of [methyl-3H]dTTP into DNA by 16% at 20 min. The release of radioactivity from labeled DNA was suppressed simultaneously. An RNase preparation from rat peritoneal macrophages enhanced the release of radioactivity from labeled DNA similarly as the soluble fraction from untreated subcellular particles of macrophages. The results suggest that the effects of the soluble fractions upon DNA metabolism of granuloma cells are at least partly independent of the effects on RNA metabolism and that the soluble fraction from SiO2-treated subcellular particles of macrophages stabilizes DNA through inhibition of nuclease activity.
从增殖性肉芽组织中分离出的细胞核，在放射性核酸前体存在的情况下，与来自未处理和经二氧化硅处理的大鼠腹膜巨噬细胞亚细胞颗粒的20000g上清液一起孵育。经二氧化硅处理的亚细胞颗粒的上清液在5分钟时使[3H]CTP最大程度地掺入核RNA增加了26%，在20分钟时使[甲基-3H]dTTP掺入DNA增加了16%。同时，标记DNA的放射性释放受到抑制。来自大鼠腹膜巨噬细胞的核糖核酸酶制剂与来自未处理的巨噬细胞亚细胞颗粒的可溶性部分类似，增强了标记DNA的放射性释放。结果表明，可溶性部分对肉芽肿细胞DNA代谢的影响至少部分独立于对RNA代谢的影响，并且来自经二氧化硅处理的巨噬细胞亚细胞颗粒的可溶性部分通过抑制核酸酶活性来稳定DNA。
