Nishimura H, Tachibana M
Hear Res. 1985 Jan;17(1):37-40. doi: 10.1016/0378-5955(85)90127-3.
A biochemical study revealed that [3H]glycerol was incorporated into various kinds of phospholipids in the guinea pig cochlea. The sites of incorporation of [3H]glycerol injected into the scala tympani were localized autoradiographically. The most active incorporation occurred in the hair cells, especially in the outer ones, followed by the inner ones and the nerve endings attached to hair cells. Glycerol incorporation into hair cells appeared to increase as the apex was approached. Chronic intoxication with kanamycin selectively suppressed glycerol incorporation in the hair cells. The site of glycerol incorporation shown by the present autoradiographic study is likely to reflect the site of phospholipid synthesis. Autoradiographic studies could constitute a new approach to understanding the possible involvement of phospholipid metabolism in the function and pathology of the cochlea.
一项生化研究表明，[3H]甘油被掺入豚鼠耳蜗的各种磷脂中。向鼓阶注射[3H]甘油后的掺入位点通过放射自显影进行定位。最活跃的掺入发生在毛细胞中，尤其是外毛细胞，其次是内毛细胞和附着于毛细胞的神经末梢。随着靠近蜗尖，甘油掺入毛细胞的情况似乎增加。卡那霉素慢性中毒选择性地抑制了毛细胞中的甘油掺入。本放射自显影研究显示的甘油掺入位点可能反映了磷脂合成的位点。放射自显影研究可能构成一种新方法，以了解磷脂代谢在耳蜗功能和病理中的可能作用。
