使用基因编码钙指示剂对皮质感觉图动态进行慢性成像。
Chronic imaging of cortical sensory map dynamics using a genetically encoded calcium indicator.
机构信息
Brain Research Institute, Department of Neurophysiology, University of Zurich, Switzerland.
出版信息
J Physiol. 2012 Jan 1;590(1):99-107. doi: 10.1113/jphysiol.2011.219014. Epub 2011 Nov 14.
Abstract
In vivo optical imaging can reveal the dynamics of large-scale cortical activity, but methods for chronic recording are limited. Here we present a technique for long-term investigation of cortical map dynamics using wide-field ratiometric fluorescence imaging of the genetically encoded calcium indicator (GECI) Yellow Cameleon 3.60. We find that wide-field GECI signals report sensory-evoked activity in anaesthetized mouse somatosensory cortex with high sensitivity and spatiotemporal precision, and furthermore, can be measured repeatedly in separate imaging sessions over multiple weeks. This method opens new possibilities for the longitudinal study of stability and plasticity of cortical sensory representations.
摘要
在体光学成像是揭示大规模皮质活动动态的有力手段，但慢性记录方法有限。本文作者展示了一种利用基因编码钙指示剂（GECI）Yellow Cameleon 3.60 的宽场比率荧光成像技术，用于长期研究皮质图谱动态。研究发现，宽场 GECI 信号以高灵敏度和时空精度报告麻醉小鼠体感皮层的感觉诱发活动，并且可以在数周的多个单独成像过程中重复测量。这种方法为皮质感觉代表的稳定性和可塑性的纵向研究开辟了新的可能性。
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