Alltech Bioscience Centre, Sarney, Dunboyne, Co. Meath, Ireland.
J Inorg Biochem. 2011 Dec;105(12):1656-61. doi: 10.1016/j.jinorgbio.2011.07.016. Epub 2011 Jul 29.
Copper(II) complexes of di-, tri- and tetra peptides with previously published protonation constants were re-investigated using pH and copper ion selective electrode (ISE) potentiometry in conjunction with a modified version of HYPERQUAD computer program. The purpose was to demonstrate the suitability of the ISE approach for the determination of apparent stability constants for copper(II) complexes with ligands for which proton stability constants were not available. The interactions of Cu(2+) with oligopeptides were also analysed using surface enhanced laser desorption/ionisation time-of-flight mass spectrometry (SELDI-ToF-MS). The results provide an insight into the metal complex species formed, their apparent stabilities under selected conditions and the effect of the relative positions of certain amino acids within the peptide sequence.
使用 pH 值和铜离子选择电极 (ISE) 电位滴定法，结合 HYPERQUAD 计算机程序的修改版本，重新研究了具有先前公布的质子化常数的二肽、三肽和四肽的铜（II）配合物。目的是证明 ISE 方法适用于测定没有质子稳定常数的配体与铜（II）配合物的表观稳定常数。使用表面增强激光解吸/电离飞行时间质谱 (SELDI-ToF-MS) 还分析了 Cu(2+) 与寡肽的相互作用。结果深入了解了形成的金属配合物种类、在选定条件下的表观稳定性以及肽序列中某些氨基酸的相对位置的影响。
