Graduate School of Medicine and Pharmaceutical Sciences for Research, University of Toyama, Sugitani, Toyama, Japan.
Int J Biol Macromol. 2012 Jan 1;50(1):245-9. doi: 10.1016/j.ijbiomac.2011.10.022. Epub 2011 Nov 6.
Four neutral polysaccharides (BRN-1, BRN-2, BRN-3 and BRN-4) were isolated from the hot water extract of the aerial part of Basella rubra L. They were found to consist of a large amount of D-galactose (81.0-92.4%) and small amounts of L-arabinose (5.4-7.8%), D-glucose (2.2-11.0%) and mannose (~2.9%). Linkage analysis revealed that all these neutral polysaccharides might be arabinogalactan type I polysaccharides in different molecular weight and chain length. Among them, only BRN-3 showed antiviral activity against herpes simplex virus type 2 (HSV-2) with 50% inhibitory concentration of 55 μg/mL without showing the cytotoxicity up to 2300 μg/mL. Furthermore, the main antiviral target of BRN-3 was shown to be the inhibition of virus adsorption to host cells. This is the first report on the neutral polysaccharide with anti-HSV-2 activity obtained from B. rubra L.
从落葵的水提物中分离得到 4 种中性多糖（BRN-1、BRN-2、BRN-3 和 BRN-4）。它们由大量的 D-半乳糖（81.0-92.4%）和少量的 L-阿拉伯糖（5.4-7.8%）、D-葡萄糖（2.2-11.0%）和甘露糖（~2.9%）组成。连接分析表明，所有这些中性多糖可能是不同分子量和链长的阿拉伯半乳聚糖 I 型多糖。其中，只有 BRN-3 对单纯疱疹病毒 2（HSV-2）具有抗病毒活性，其 50%抑制浓度为 55 μg/mL，在 2300 μg/mL 时没有显示细胞毒性。此外，BRN-3 的主要抗病毒靶标被证明是抑制病毒吸附到宿主细胞。这是首次从落葵属植物中分离得到具有抗 HSV-2 活性的中性多糖。
