Manosroi Aranya, Pattamapun Kassara, Chankhampan Charinya, Kietthanakorn Bang-On, Kitdamrongtham Worapong, Zhang Jie, Manosroi Jiradej
Manose Health and Beauty Research Center, Mueang, Chiang Mai 50200, Thailand.
Faculty of Engineering and Technology, North-Chiang Mai University, Chiang Mai 50230, Thailand.
Saudi J Biol Sci. 2020 Mar;27(3):769-776. doi: 10.1016/j.sjbs.2020.01.007. Epub 2020 Jan 16.
Ceylon Spinach () is an edible perennial vine found in tropical Asia and Africa, known as vegetables containing mucilage. Its mucilage from flowers was extracted by microwaving and precipitated with 95% ethanol. Five artificial saliva formulations composing of mucilage from Ceylon Spinach, calcium chloride (CaCl), potassium chloride (KCl) and sodium fluoride (NF) were developed. The best formulation No.5 containing 0.61% of the mucilage with the non-Newtonian pseudoplastic flow (8.9 ± 0.2 cP) and the wetting time (12.50 ± 2.24 min) similar to the normal human saliva was selected. This artificial saliva formulation exhibited biological activities including an antioxidative activity by DPPH free radical scavenging with the SC of 14.26 ± 2.00 mg/ml (0.05 folds of ascorbic acid), and the adhesion inhibition of on hydroxyapatite beads at 17.01 ± 7.75%, while the natural human saliva exhibited an increase bacterial adhesion of 33.10 ± 9.70%. The safety of this formulation which gave no cytotoxicity on normal human gingival fibroblasts at 99.20 ± 21.09% cell viability was also demonstrated. The results from this study have indicated high biological activity and safety of the developed formulation containing mucilage from Ceylon Spinach which is potential to be used as artificial saliva for xerostomia patients.
锡兰菠菜是一种可食用的多年生藤本植物，生长于热带亚洲和非洲，是一种富含黏液质的蔬菜。其花中的黏液质通过微波提取并用95%乙醇沉淀。制备了五种由锡兰菠菜黏液质、氯化钙（CaCl）、氯化钾（KCl）和氟化钠（NF）组成的人工唾液配方。选择了最佳配方5，其含有0.61%的黏液质，具有与正常人唾液相似的非牛顿假塑性流动（8.9±0.2厘泊）和润湿时间（12.50±2.24分钟）。这种人工唾液配方具有生物活性，包括通过清除DPPH自由基表现出抗氧化活性，其清除能力为14.26±2.00毫克/毫升（是抗坏血酸的0.05倍），对羟基磷灰石珠的黏附抑制率为17.01±7.75%，而正常人唾液的细菌黏附增加率为33.10±9.70%。还证明了该配方的安全性，其对正常人牙龈成纤维细胞无细胞毒性，细胞活力为99.20±21.09%。这项研究的结果表明，所开发的含有锡兰菠菜黏液质的配方具有高生物活性和安全性，有潜力用作口干患者的人工唾液。
