Yan J Z, Su T C
Department of Parasitology, Hubei Medical College.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1990;8(2):121-3.
To compare the effects of sera on the growth of Plasmodium berghei, the erythrocytic stages were cultured with rat serum, human umbilical cord serum, human B-type serum, rabbit serum, calf serum and calf serum with hypoxanthine respectively. The topography of the erythrocyte and parasite cultured with calf serum were observed before and 12, 20 and 28 hours after cultivation. All of the sera used did not effectively improve the long-term culture of P. berghei, regardless of some differences in short-term cultures. Under SEM, erythrocytes agglutinated with each other and the surface adhesive materials on the erythrocytes and parasites agglomerated gradually with the cultivation time. The results suggested that the surface adhesive materials were correlated with the destruction of erythrocytes, malnutrition of the parasites and blockade of merozoite reinvasion.
为比较不同血清对伯氏疟原虫生长的影响，分别用大鼠血清、人脐带血清、人B型血清、兔血清、小牛血清以及添加次黄嘌呤的小牛血清培养疟原虫的红细胞内期。在培养前以及培养12、20和28小时后，观察用小牛血清培养的红细胞和疟原虫的形态。所用的所有血清均未有效改善伯氏疟原虫的长期培养，尽管短期培养存在一些差异。在扫描电子显微镜下，红细胞相互凝集，红细胞和疟原虫表面的黏附物质随着培养时间逐渐聚集。结果表明，表面黏附物质与红细胞破坏、疟原虫营养不良以及裂殖子再侵入受阻有关。
