锥虫与一个 50 年线粒体钙奥秘的解决方案。
Trypanosomes and the solution to a 50-year mitochondrial calcium mystery.
机构信息
Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, GA 30620, USA.
出版信息
Trends Parasitol. 2012 Jan;28(1):31-7. doi: 10.1016/j.pt.2011.10.007. Epub 2011 Nov 14.
Abstract
The ability of mitochondria to take up Ca(2+) was discovered 50 years ago. This calcium uptake, through a mitochondrial calcium uniporter (MCU), is important not only for the regulation of cellular ATP concentration but also for more complex pathways such as shaping Ca(2+) signals and the activation of programmed cell death. The molecular nature of the uniporter remained unknown for decades. By a comparative study of mitochondrial protein profiles of organisms lacking or possessing MCU, such as yeast in the former case and vertebrates and trypanosomes in the latter, two groups recently found the protein that possesses all the characteristics of the MCU. These results add another success story to the already substantial contributions of trypanosomes to mammalian biochemistry.
摘要
线粒体摄取 Ca(2+)的能力在 50 年前就被发现了。这种通过线粒体钙离子单向转运体（MCU）的钙摄取，不仅对细胞 ATP 浓度的调节很重要，而且对更复杂的途径（如塑造 Ca(2+)信号和激活程序性细胞死亡）也很重要。单向转运体的分子性质在几十年里一直不为人知。通过对缺乏或拥有 MCU 的生物体（如前者的酵母和后者的脊椎动物和锥虫）的线粒体蛋白图谱进行比较研究，最近两组研究人员发现了一种具有 MCU 所有特征的蛋白质。这些结果为锥虫对哺乳动物生物化学的已有重大贡献再添一个成功案例。
相似文献
1
Trypanosomes and the solution to a 50-year mitochondrial calcium mystery.锥虫与一个 50 年线粒体钙奥秘的解决方案。
Trends Parasitol. 2012 Jan;28(1):31-7. doi: 10.1016/j.pt.2011.10.007. Epub 2011 Nov 14.
2
The mitochondrial calcium uniporter complex in trypanosomes.线粒体钙单向转运蛋白复合物在锥虫中的作用。
Cell Biol Int. 2018 Jun;42(6):656-663. doi: 10.1002/cbin.10928. Epub 2018 Jan 25.
3
Mitochondrial Ca homeostasis in trypanosomes.线粒体钙离子稳态在锥虫中的作用。
Int Rev Cell Mol Biol. 2021;362:261-289. doi: 10.1016/bs.ircmb.2021.01.002. Epub 2021 Feb 27.
4
The Mitochondrial Calcium Uniporter Interacts with Subunit c of the ATP Synthase of Trypanosomes and Humans.线粒体钙单向转运蛋白与原生动物和人类的 ATP 合酶亚基 c 相互作用。
mBio. 2020 Mar 17;11(2):e00268-20. doi: 10.1128/mBio.00268-20.
5
Mitochondrial calcium transport in trypanosomes.锥虫中的线粒体钙转运
Mol Biochem Parasitol. 2014 Sep;196(2):108-16. doi: 10.1016/j.molbiopara.2014.09.001. Epub 2014 Sep 10.
6
The Mitochondrial Ca Uniporter Complex (MCUC) of Trypanosoma brucei Is a Hetero-oligomer That Contains Novel Subunits Essential for Ca Uptake.布氏锥虫的线粒体钙单向转运体复合物（MCUC）是一种异源寡聚体，包含对钙摄取至关重要的新型亚基。
mBio. 2018 Sep 18;9(5):e01700-18. doi: 10.1128/mBio.01700-18.
7
[The Molecular Mechanisms of Mitochondrial Calcium Uptake by Calcium Uniporter].[钙单向转运体介导线粒体钙摄取的分子机制]
Yakugaku Zasshi. 2021;141(4):491-499. doi: 10.1248/yakushi.20-00204-1.
8
MCU-i4, a mitochondrial Ca uniporter modulator, induces breast cancer BT474 cell death by enhancing glycolysis, ATP production and reactive oxygen species (ROS) burst.MCU-i4是一种线粒体钙单向转运体调节剂，通过增强糖酵解、ATP生成和活性氧（ROS）爆发来诱导乳腺癌BT474细胞死亡。
Oncol Res. 2025 Jan 16;33(2):397-406. doi: 10.32604/or.2024.052743. eCollection 2025.
9
Different Roles of Mitochondrial Calcium Uniporter Complex Subunits in Growth and Infectivity of .线粒体钙单向转运体复合物亚基在……生长和感染性中的不同作用
mBio. 2017 May 9;8(3):e00574-17. doi: 10.1128/mBio.00574-17.
10
The Mitochondrial Calcium Uniporter Selectively Matches Metabolic Output to Acute Contractile Stress in the Heart.线粒体钙单向转运体使心脏代谢输出与急性收缩应激选择性匹配。
Cell Rep. 2015 Jul 7;12(1):15-22. doi: 10.1016/j.celrep.2015.06.002. Epub 2015 Jun 25.
引用本文的文献
1
Massive Accumulation of Strontium and Barium in Diplonemid Protists.厚壁网菌属原生生物中锶和钡的大量积累。
mBio. 2023 Feb 28;14(1):e0327922. doi: 10.1128/mbio.03279-22. Epub 2023 Jan 16.
2
MICU1 and MICU2 potentiation of Ca uptake by the mitochondrial Ca uniporter of Trypanosoma cruzi and its inhibition by Mg.微小钙调蛋白 1（MICU1）和微小钙调蛋白 2（MICU2）增强克氏锥虫线粒体钙单向转运体摄取 Ca2+的作用及其被镁抑制。
Cell Calcium. 2022 Nov;107:102654. doi: 10.1016/j.ceca.2022.102654. Epub 2022 Sep 21.
3
Mitochondrial Ca homeostasis in trypanosomes.线粒体钙离子稳态在锥虫中的作用。
Int Rev Cell Mol Biol. 2021;362:261-289. doi: 10.1016/bs.ircmb.2021.01.002. Epub 2021 Feb 27.
4
Mitochondrial Ca and Reactive Oxygen Species in Trypanosomatids.线粒体钙和原生动物中的活性氧物质。
Antioxid Redox Signal. 2022 May;36(13-15):969-983. doi: 10.1089/ars.2021.0058. Epub 2021 Sep 17.
5
Trypanosoma cruzi Letm1 is involved in mitochondrial Ca transport, and is essential for replication, differentiation, and host cell invasion.克氏锥虫Letm1参与线粒体钙运输，对复制、分化和宿主细胞入侵至关重要。
FASEB J. 2021 Jul;35(7):e21685. doi: 10.1096/fj.202100120RR.
6
Intracellular Ca Signaling in Protozoan Parasites: An Overview with a Focus on Mitochondria.原生动物寄生虫细胞内 Ca 信号转导：线粒体为重点的概述。
Int J Mol Sci. 2021 Jan 5;22(1):469. doi: 10.3390/ijms22010469.
7
The Mitochondrial Calcium Uniporter Interacts with Subunit c of the ATP Synthase of Trypanosomes and Humans.线粒体钙单向转运蛋白与原生动物和人类的 ATP 合酶亚基 c 相互作用。
mBio. 2020 Mar 17;11(2):e00268-20. doi: 10.1128/mBio.00268-20.
8
Functional analysis and importance for host cell infection of the Ca-conducting subunits of the mitochondrial calcium uniporter of .线粒体钙单向转运体的 Ca 电导亚基的功能分析及其对宿主细胞感染的重要性
Mol Biol Cell. 2019 Jul 1;30(14):1676-1690. doi: 10.1091/mbc.E19-03-0152. Epub 2019 May 15.
9
MICU1 and MICU2 Play an Essential Role in Mitochondrial Ca Uptake, Growth, and Infectivity of the Human Pathogen Trypanosoma cruzi.MICU1 和 MICU2 在人病原体克氏锥虫的线粒体钙摄取、生长和感染性中发挥重要作用。
mBio. 2019 May 7;10(3):e00348-19. doi: 10.1128/mBio.00348-19.
10
Calcium signaling and the lytic cycle of the Apicomplexan parasite Toxoplasma gondii.钙信号转导与顶复门原虫弓形虫的裂解周期。
Biochim Biophys Acta Mol Cell Res. 2018 Nov;1865(11 Pt B):1846-1856. doi: 10.1016/j.bbamcr.2018.08.004. Epub 2018 Aug 10.
本文引用的文献
1
Stage-specific requirement for Isa1 and Isa2 proteins in the mitochondrion of Trypanosoma brucei and heterologous rescue by human and Blastocystis orthologues.伊萨蛋白 1（Isa1）和伊萨蛋白 2（Isa2）在布氏锥虫线粒体中的阶段特异性要求，以及人源和人芽囊原虫同源物的异源拯救。
Mol Microbiol. 2011 Sep;81(6):1403-18. doi: 10.1111/j.1365-2958.2011.07769.x. Epub 2011 Aug 15.
2
A forty-kilodalton protein of the inner membrane is the mitochondrial calcium uniporter.一种四十千道尔顿的内膜蛋白是线粒体钙单向转运蛋白。
Nature. 2011 Jun 19;476(7360):336-40. doi: 10.1038/nature10230.
3
Integrative genomics identifies MCU as an essential component of the mitochondrial calcium uniporter.整合基因组学鉴定 MCU 为线粒体钙单向转运体的必需组成部分。
Nature. 2011 Jun 19;476(7360):341-5. doi: 10.1038/nature10234.
4
Molecules and roles of mitochondrial calcium signaling.线粒体钙信号的分子与作用。
Biofactors. 2011 May-Jun;37(3):219-27. doi: 10.1002/biof.160. Epub 2011 Jun 14.
5
Trypanosomatid apoptosis: 'Apoptosis' without the canonical regulators.锥体虫细胞凋亡：没有典型调控因子的“凋亡”。
Virulence. 2011 May-Jun;2(3):253-6. doi: 10.4161/viru.2.3.16278. Epub 2011 May 1.
6
Mitochondrial translation in trypanosomatids: a novel target for chemotherapy?锥虫线粒体翻译：化疗新靶点？
Trends Parasitol. 2011 Oct;27(10):429-33. doi: 10.1016/j.pt.2011.03.011. Epub 2011 Apr 30.
7
Evolution of apoptosis-like programmed cell death in unicellular protozoan parasites.单细胞原生动物寄生虫中凋亡样程序性细胞死亡的进化。
Parasit Vectors. 2011 Mar 25;4:44. doi: 10.1186/1756-3305-4-44.
8
Identification of contractile vacuole proteins in Trypanosoma cruzi.鉴定克氏锥虫的收缩泡蛋白。
PLoS One. 2011 Mar 18;6(3):e18013. doi: 10.1371/journal.pone.0018013.
9
Functional characterization of NADP-dependent isocitrate dehydrogenase isozymes from Trypanosoma cruzi.克氏锥虫NADP依赖型异柠檬酸脱氢酶同工酶的功能特性
Mol Biochem Parasitol. 2011 May;177(1):61-4. doi: 10.1016/j.molbiopara.2011.01.010. Epub 2011 Feb 1.
10
Futile import of tRNAs and proteins into the mitochondrion of Trypanosoma brucei evansi.伊氏锥虫线粒体中tRNA和蛋白质的无效输入
Mol Biochem Parasitol. 2011 Apr;176(2):116-20. doi: 10.1016/j.molbiopara.2010.12.010. Epub 2010 Dec 30.
Zotero 插件