Departament de Química, Facultat de Ciències, Universitat Autònoma de Barcelona, 08193-Cerdanyola del Vallès, Barcelona, Spain.
Dalton Trans. 2012 Jan 7;41(1):300-6. doi: 10.1039/c1dt11506e. Epub 2011 Nov 17.
The interaction of a novel Pt complex, Pt(dmba)(N9-9AA)(PPh(3))1 (dmba = N,N-dimethylbenzylamine-κN,κC; 9AA = 9-aminoacridine), which exhibits anti-tumor activity, with certain key proteins has been monitored by ESI-MS. Also, the interaction of 1 with a designed double-stranded oligonucleotide containing the GG motif has been followed by mass spectrometry as well as by fluorimetry. The results obtained show the low interaction of 1 with the considered proteins and the absence of covalent interaction with the oligonucleotides, but the fluorimetric data confirm the π-π interaction of 1 with the double-stranded DNA, which is probably the reason of the previously reported activity of 1 in several tumor cell lines.
新型 Pt 配合物Pt(dmba)(N9-9AA)(PPh(3))1（dmba=N,N-二甲基苄基胺-κN，κC；9AA=9-氨基吖啶）与某些关键蛋白的相互作用已通过 ESI-MS 进行了监测。此外，还通过质谱法和荧光法研究了 1 与含有 GG 基序的设计双链寡核苷酸的相互作用。所获得的结果表明，1 与所考虑的蛋白质的相互作用较弱，并且与寡核苷酸没有共价相互作用，但荧光数据证实了 1 与双链 DNA 的π-π相互作用，这可能是 1 在几种肿瘤细胞系中先前报道的活性的原因。
