与基于胆碱的慢速冷冻相比，使用 Cryoleaf 玻璃化法对不成熟的人类卵母细胞进行冷冻保存可改善其成熟结局。
Maturation outcomes are improved following Cryoleaf vitrification of immature human oocytes when compared to choline-based slow-freezing.
机构信息
Biology Department, Middlebury College, Middlebury, VT 05753, USA.
出版信息
J Assist Reprod Genet. 2011 Dec;28(12):1183-92. doi: 10.1007/s10815-011-9674-x. Epub 2011 Nov 17.
Abstract
PURPOSE
The cryopreservation of immature oocytes permits oocyte banking for patients at risk of losing their fertility. However, the optimum protocol for such fertility preservation remains uncertain.
METHODS
The present study investigated the survival, maturation, cytoskeletal and chromosome organization of sibling immature oocytes leftover from controlled ovarian stimulation cycles, that were either slow-frozen (with choline-substitution) or vitrified. A comparison group included oocytes that were never cryopreserved.
RESULTS
Among the three groups, comparable rates were observed for both survival (67-70%) and polar body extrusion (59-79%). Significantly more oocytes underwent spontaneous activation after IVM following slow-freezing compared with either vitrification or no cryopreservation. Likewise, the incidence of spindle abnormalities was greatest in the slow-frozen group, with no differences in spindle morphometrics or chromosome organization.
CONCLUSIONS
While the overall incidence of mature oocytes with normal bipolar spindles from warmed immature oocytes was low, the yield using Cryoleaf vitrification was slightly superior to choline-based slow-freezing.
摘要
目的
不成熟卵母细胞的冷冻保存允许对有生育能力丧失风险的患者进行卵母细胞储存。然而，这种生育力保存的最佳方案仍不确定。
方法
本研究调查了来自控制性卵巢刺激周期的剩余姐妹不成熟卵母细胞的存活、成熟、细胞骨架和染色体结构，这些卵母细胞要么进行慢速冷冻（用胆碱替代），要么进行玻璃化冷冻。比较组包括从未进行过冷冻保存的卵母细胞。
结果
在三组中，存活（67-70%）和极体排出（59-79%）的比例相似。与玻璃化或未冷冻保存相比，慢速冷冻后 IVM 后自发激活的卵母细胞明显更多。同样，在慢速冷冻组中，纺锤体异常的发生率最高，而在纺锤体形态计量或染色体结构方面没有差异。
结论
尽管从解冻的不成熟卵母细胞中获得具有正常双极纺锤体的成熟卵母细胞的总体发生率较低，但使用 Cryoleaf 玻璃化冷冻的产率略优于基于胆碱的慢速冷冻。
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