School of Life Science, Huzhou University, Huzhou, 313000, Zhejiang, China.
Zhejiang Provincial Key Laboratory of Aquatic Resource Conservation and Development, Huzhou University, Huzhou, 313000, Zhejiang, China.
World J Microbiol Biotechnol. 2021 Jan 4;37(1):8. doi: 10.1007/s11274-020-02974-4.
Dye-decolorization is one of the most important steps in dye-polluted wastewater treatment. The dye-decolorization bacteria were isolated from active sludge collected from wastewater treating pond of a dyeing and printing plant using serial dilution method. Among the 44 bacteria isolates from the active sludge, the strain Bacillus amyloliquefaciens W36 was found to have strong ability in dye-decolorization. The effects of carbon source, nitrogen sources, C/N, metal ions, temperature, pH, and rotation speed for dye-decolorization were investigated. The optimum decolorization conditions were that the strain was grown in enriched mineral salt medium (EMSM) using maltose 1 g/L, (NH)SO 1 g/L as carbon and nitrogen source respectively, supplemented with 100 mg/L different dyes (pH 6.0), at 30 °C, 200 rpm from 48 to 96 h. The bacteria could aerobically decolorize dyes, such as Coomassie brilliant blue (95.42%), Bromcresol purple (93.34%), Congo red (72.37%) and Sarranine (61.7%), within 96 h. The dyes decolorization products were analyzed by ultra-violet and visible (UV-vis) spectroscopy before and after decolorization, which indicated that the four dyes were significantly degraded by the strain. The results indicated that the bacteria Bacillus amyloliquefaciens W36 could be used in dye-polluted wastewater treatment.
脱色是处理染料污染废水的最重要步骤之一。采用连续稀释法从印染厂废水处理池的活性污泥中分离出染料脱色细菌。在从活性污泥中分离出的 44 株细菌中，发现解淀粉芽孢杆菌 W36 具有很强的染料脱色能力。研究了碳源、氮源、C/N、金属离子、温度、pH 值和转速对染料脱色的影响。最佳脱色条件为在富集无机盐培养基（EMSM）中，以麦芽糖 1 g/L 和（NH4）2SO4 1 g/L 分别作为碳源和氮源，在 pH 值为 6.0 的条件下，添加 100 mg/L 的不同染料（培养温度 30°C，转速 200 rpm），培养 48-96 h。细菌可以在 96 h 内好氧脱色染料，如考马斯亮蓝（95.42%）、溴甲酚绿（93.34%）、刚果红（72.37%）和沙仑（61.7%）。在脱色前后通过紫外可见（UV-vis）光谱分析染料脱色产物，表明四种染料均被该菌显著降解。结果表明，解淀粉芽孢杆菌 W36 可用于处理染料污染废水。
