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在碱性 pH 值下的碱基识别和固定在生物纳米孔内的单个 DNA 碱基的表观 pKa。

Nucleobase recognition at alkaline pH and apparent pKa of single DNA bases immobilised within a biological nanopore.

机构信息

Department of Chemistry, University of Leuven, 3001, Heverlee, Leuven, Belgium.

出版信息

Chem Commun (Camb). 2012 Feb 1;48(10):1520-2. doi: 10.1039/c1cc16124e. Epub 2011 Nov 17.

DOI:10.1039/c1cc16124e
PMID:22089628
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3393037/
Abstract

The four DNA bases are recognized in immobilized DNA strands at high alkaline pH by nanopore current recordings. Ionic currents through the biological nanopores are also employed to measure the apparent pK(a) values of single nucleobases within the immobilised DNA strands.

摘要

在高碱性 pH 值下，通过纳米孔电流记录可识别固定化 DNA 链中的四个 DNA 碱基。通过生物纳米孔的离子电流也被用于测量固定化 DNA 链中单核碱基的表观 pK(a) 值。

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