State Key Laboratory of Chemical Resource Engineering, Analytical Chemistry Department, School of Science, Beijing University of Chemical Technology, Beijing 100029, People's Republic of China.
Anal Chem. 2012 Jan 3;84(1):386-95. doi: 10.1021/ac202735v. Epub 2011 Dec 14.
Quantum dots (QDs)-based molecularly imprinted polymer (MIP) composite nanospheres were successfully prepared via a facile and versatile ultrasonication-assisted encapsulation method. Unlike the hydrogen-bond-based MIPs, these so-prepared QDs-MIP composite nanospheres, relying on the interaction including van der Waals forces and hydrophobic forces, demonstrated excellent selectivity in aqueous media. Their small particle sizes and carboxyl-enriched polymer matrixes give rise to their good dispersibility and stability in aqueous solution, and faster adsorption and desorption kinetics, which further make them extensively applicable for chemical/biological sensors in aqueous media. Based on the fluorescence quenching via template analytes (diazinon) rebinding into the recognition cavities in the polymer matrixes, the QDs-MIP nanospheres were successfully applied to the direct fluorescence quantification of diazinon, independent of extracting templates from the MIP nanospheres, as well as further complicated and time-consuming assays. This novel method can selectively and sensitively detect down to 50 ng/mL of diazinon in water, and a linear relationship has been obtained covering the concentration range of 50-600 ng/mL. The present studies provide a new and general strategy to fabricate other multifunctional (luminescent and magnetic) inorganic-organic MIP nanocomposites with highly selective recognition ability in aqueous media and are pretty desirable for biomedical/chemical sensing applications.
量子点（QD）基分子印迹聚合物（MIP）复合纳米球通过简便且通用的超声辅助包封方法成功制备。与基于氢键的 MIP 不同，这些通过预制备的 QD-MIP 复合纳米球，依靠包括范德华力和疏水力在内的相互作用，在水介质中表现出优异的选择性。它们的小颗粒尺寸和富含羧基的聚合物基质赋予其在水溶液中的良好分散性和稳定性，以及更快的吸附和解吸动力学，这进一步使得它们广泛适用于水介质中的化学/生物传感器。基于模板分析物（二嗪农）通过重新结合到聚合物基质中的识别腔中来进行荧光猝灭，QD-MIP 纳米球成功地应用于直接荧光定量检测二嗪农，无需从 MIP 纳米球中提取模板，以及进一步的复杂和耗时的测定。该新型方法可以选择性和灵敏地检测水中低至 50ng/mL 的二嗪农，并且在 50-600ng/mL 的浓度范围内获得了线性关系。目前的研究为在水介质中具有高选择性识别能力的其他多功能（发光和磁性）无机-有机 MIP 纳米复合材料的制备提供了一种新的通用策略，非常适合生物医学/化学传感应用。
