Department of Chemistry, College of Science, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China.
Department of Chemistry, College of Science, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China.
Biosens Bioelectron. 2015 Feb 15;64:182-8. doi: 10.1016/j.bios.2014.08.086. Epub 2014 Sep 6.
A newly designed molecularly imprinted polymer (MIP) material was fabricated and successfully utilized as recognition element to develop a quantum dots (QDs) based MIP-coated composite for selective recognition of the template cyphenothrin. The MIP-coated QDs were characterized by fluorescence spectrophotometer, Fourier transform infrared spectroscopy, transmission electron microscope, dynamic light scattering and X-ray powder diffraction. The fluorescence of the coated QDs is quenched on loading the MIP with cyphenothrin, and the effect is much stronger for the MIP than for the non-imprinted polymer, which indicates the MIP could as a recognition template composite. This method can detect down to 9.0 nmol L(-1) of cyphenothrin in water, and a linear relationship has been obtained covering the concentration range of 0.1-80.0 μmol L(-1). The method has been used in the determination of cyphenothrin in water samples and gave recoveries in the range from 88.5% to 97.1% with relative standard deviations in the range of 3.1-6.2%. The present study provides a new and general strategy to fabricate inorganic-organic MIP-coated QDs with highly selective recognition ability in aqueous media and is desirable for chemical probe application.
一种新型的分子印迹聚合物（MIP）材料被设计并成功地用作识别元件，用于开发基于量子点（QDs）的 MIP 涂层复合材料，以选择性识别模板氯菊酯。通过荧光分光光度计、傅里叶变换红外光谱、透射电子显微镜、动态光散射和 X 射线粉末衍射对 MIP 涂层 QDs 进行了表征。当 MIP 与氯菊酯一起加载时，涂层 QDs 的荧光被猝灭，并且 MIP 的猝灭效果比非印迹聚合物强得多，这表明 MIP 可以作为识别模板复合材料。该方法可在水中检测到低至 9.0 nmol L(-1)的氯菊酯，并且在 0.1-80.0 μmol L(-1)的浓度范围内得到了线性关系。该方法已用于水样中氯菊酯的测定，回收率在 88.5%至 97.1%之间，相对标准偏差在 3.1%至 6.2%之间。本研究为在水相介质中制备具有高选择性识别能力的无机-有机 MIP 涂层 QDs 提供了一种新的通用策略，有望用于化学探针应用。
