State Key Laboratory of Electroanalytical Chemistry, c/o Engineering Laboratory for Modern Analytical Techniques, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China.
Biosens Bioelectron. 2012 Oct-Dec;38(1):55-60. doi: 10.1016/j.bios.2012.04.043. Epub 2012 May 10.
A new type of eco-friendly molecularly imprinted polymer (MIP) was synthesized through an efficient one-pot room-temperature sol-gel polymerization and applied as a molecular recognition element to construct dopamine (DA) fluorescence (FL) optosensor. Highly luminescent carbon dots (CDs) were firstly synthesized via a one-step reaction in organosilane, and their surface were anchored with MIP matrix (CDs@MIP). The resulting composite of a synergetic combination of CDs with MIP showed high photostability and template selectivity. Moreover, the composite allowed a highly sensitive determination of DA via FL intensity decreasing when removal of the original templates. The new MIP-based DA sensing protocol was applied to detect DA concentration in aqueous solution, the relative FL intensity of CDs@MIP decreased linearly with the increasing DA in the concentration range of 25-500nM with a detection limit (3σ) of 1.7 nM. Furthermore, the proposed method was successfully intended for the determination of trace DA in human urine samples without the interference of other molecules and ions.
一种新型的环保型分子印迹聚合物（MIP）通过高效的一锅室温溶胶-凝胶聚合反应合成，并作为分子识别元件用于构建多巴胺（DA）荧光（FL）光传感器。首先通过在有机硅烷中的一步反应合成了高发光的碳点（CDs），并将其表面固定在 MIP 基质上（CDs@MIP）。CDs 与 MIP 的协同组合的复合材料表现出高的光稳定性和模板选择性。此外，当去除原始模板时，复合材料通过 FL 强度的降低允许对 DA 进行高灵敏度的测定。新的基于 MIP 的 DA 传感方案被应用于检测水溶液中的 DA 浓度，CDs@MIP 的相对 FL 强度随 DA 在 25-500nM 浓度范围内的增加呈线性下降，检测限（3σ）为 1.7 nM。此外，该方法成功地用于测定人尿液样品中的痕量 DA，没有其他分子和离子的干扰。
