Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand.
FEMS Microbiol Ecol. 2012 Feb;79(2):273-81. doi: 10.1111/j.1574-6941.2011.01216.x. Epub 2011 Oct 24.
This study investigated bacterial community structures in the midguts of Apis mellifera and Apis cerana in Thailand to understand how bacterial communities develop in Apis species. The bacterial species present in replicate colonies from different locations and life stages were analysed. PCR amplification of bacterial 16S rRNA gene fragments and terminal restriction fragment length polymorphism analyses revealed a total of 16 distinct terminal restriction fragments (T-RFs), 12 of which were shared between A. mellifera and A. cerana populations. The T-RFs were affiliated to Beta- and Gammaproteobacteria, Firmicutes and Actinomycetes. The Gammaproteobacteria were found to be common in all stages of honey bee, but in addition, the Firmicutes group was found to be present in the worker bees. Bacterial community structure showed no difference amongst the replicate colonies, but was affected to some degree by geographical location, life stage and species of honey bees.
本研究旨在调查泰国地区的西方蜜蜂和中华蜜蜂的中肠细菌群落结构，以了解蜜蜂物种中细菌群落的发展方式。分析了来自不同地点和生活阶段的重复蜂群中的细菌种类。细菌 16S rRNA 基因片段的 PCR 扩增和末端限制性片段长度多态性分析共揭示了 16 个不同的末端限制性片段（T-RFs），其中 12 个在西方蜜蜂和中华蜜蜂种群中共有。T-RFs 与β-变形菌纲、厚壁菌门和放线菌门有关。在所有阶段的蜜蜂中都发现了γ-变形菌，此外，还发现厚壁菌门存在于工蜂中。细菌群落结构在重复蜂群中没有差异，但在一定程度上受到地理位置、生活阶段和蜜蜂物种的影响。
