Panjad Poonnawat, Yongsawas Rujipas, Sinpoo Chainarong, Pakwan Chonthicha, Subta Phakamas, Krongdang Sasiprapa, In-On Ammarin, Chomdej Siriwadee, Chantawannakul Panuwan, Disayathanoowat Terd
Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Faculty of Science and Social Sciences, Burapha University Sakaeo Campus, Sakaeo 27160, Thailand.
Insects. 2021 Jun 6;12(6):525. doi: 10.3390/insects12060525.
Honeybees, , are important pollinators of many economically important crops. However, one of the reasons for their decline is pathogenic infection. Nosema disease and American foulbrood (AFB) disease are the most common bee pathogens that propagate in the gut of honeybees. This study investigated the impact of gut-propagating pathogens, including and on bacterial communities in the gut of using 454-pyrosequencing. Pyrosequencing results showed that was implicated in the elimination of and the dramatic increase in and in adult bees' guts, while bacterial communities of infected larvae were not affected by the infection. The results indicated that only had an impact on some core bacteria in the gut of through increasing core gut bacteria, therefore leading to the induction of dysbiosis in the bees' gut.
蜜蜂是许多经济作物的重要传粉者。然而，其数量减少的原因之一是病原感染。微孢子虫病和美洲幼虫腐臭病（AFB）是在蜜蜂肠道中传播的最常见的蜜蜂病原体。本研究使用454焦磷酸测序技术，调查了包括微孢子虫和美洲幼虫芽孢杆菌在内的肠道传播病原体对意大利蜜蜂肠道细菌群落的影响。焦磷酸测序结果表明，微孢子虫与成年蜜蜂肠道中双歧杆菌的消除以及乳酸杆菌和肠球菌的急剧增加有关，而受感染幼虫的细菌群落不受感染影响。结果表明，只有美洲幼虫芽孢杆菌通过增加肠道核心细菌对意大利蜜蜂肠道中的一些核心细菌产生影响，从而导致蜜蜂肠道菌群失调。
