Department of Biochemistry and Molecular Biology, Wright State University, Dayton, OH 45435, USA.
FEMS Microbiol Ecol. 2012 Jan;79(1):2-11. doi: 10.1111/j.1574-6941.2011.01222.x. Epub 2011 Nov 9.
Human-associated microbiota is recognized to play vital roles in maintaining host health, and it is implicated in many disease states. While the initial surge in the profiling of these microbial communities was achieved with Sanger and next-generation sequencing, many oligonucleotide microarrays have also been developed recently for this purpose. Containing probes complementary to small ribosomal subunit RNA gene sequences of community members, such phylogenetic arrays provide direct quantitative comparisons of microbiota composition among samples and between sample groups. Some of the developed microarrays including PhyloChip, Microbiota Array, and HITChip can simultaneously measure the presence and abundance of hundreds and thousands of phylotypes in a single sample. This review describes the currently available phylogenetic microarrays that can be used to analyze human microbiota, delineates the approaches for the optimization of microarray use, and provides examples of recent findings based on microarray interrogation of human-associated microbial communities.
人体相关微生物群被认为在维持宿主健康方面发挥着重要作用，并且与许多疾病状态有关。虽然最初通过 Sanger 和下一代测序来对这些微生物群落进行分析，但最近也已经开发了许多寡核苷酸微阵列用于此目的。这些基于序列的微阵列包含与群落成员的小核糖体亚基 RNA 基因序列互补的探针，可直接对样品之间和样品组之间的微生物群落组成进行定量比较。一些已开发的微阵列，包括 PhyloChip、Microbiota Array 和 HITChip，可在单个样本中同时测量数百种甚至数千种生物型的存在和丰度。本综述描述了目前可用于分析人体微生物群的基于序列的微阵列，阐述了优化微阵列使用的方法，并提供了基于对人体相关微生物群落进行微阵列检测的最新发现的示例。
