Belibasakis Georgios N, Guggenheim Bernhard
Oral Microbiology and Immunology, Institute of Oral Biology, Center of Dental Medicine, University of Zürich, Switzerland.
FEMS Immunol Med Microbiol. 2011 Dec;63(3):381-6. doi: 10.1111/j.1574-695X.2011.00863.x. Epub 2011 Oct 4.
Polymicrobial oral biofilms attaching on tooth surfaces can trigger inflammatory responses by the neighbouring tooth-supporting periodontal tissues. An excessive inflammatory response can cause destruction of the periodontal tissues, including the alveolar bone, thus resulting in periodontitis. Mediators of inflammation, such as prostaglandin E(2) (PGE(2) ) and interleukin-6, are primary regulators of alveolar bone destruction in periodontitis. The present study aimed to comparatively investigate the effects of in vitro supragingival and subgingival biofilms, on the regulation of PGE(2) and interleukin-6 in human gingival fibroblasts. The cells were challenged with culture supernatants of the two biofilms for 6 h. Cyclo-oxygenase (COX)-2, an enzyme responsible for the conversion of PGE(2) , and interleukin-6 gene expression were analysed by quantitative real-time PCR. The production of PGE(2) and interleukin-6 by the cells was analysed by ELISA. While the supragingival biofilm did not induce significant changes, the subgingival biofilm caused an 8.6- and 2.9-fold enhancement of COX-2 and interleukin-6 gene expression, respectively, and a 72.5- and 1.5-fold enhancement of PGE(2) and interleukin-6 production, respectively. In conclusion, subgingival biofilms are potent inducers of PGE(2) in gingival fibroblasts, providing further mechanistic insights into the association of subgingival biofilms with bone resorption periodontitis.
附着在牙齿表面的多微生物口腔生物膜可引发邻近牙齿支持性牙周组织的炎症反应。过度的炎症反应会导致包括牙槽骨在内的牙周组织破坏，从而引发牙周炎。炎症介质，如前列腺素E(2)（PGE(2)）和白细胞介素-6，是牙周炎中牙槽骨破坏的主要调节因子。本研究旨在比较体外龈上和龈下生物膜对人牙龈成纤维细胞中PGE(2)和白细胞介素-6调节的影响。用两种生物膜的培养上清液刺激细胞6小时。通过定量实时PCR分析负责PGE(2)转化的环氧化酶（COX）-2和白细胞介素-6基因表达。通过ELISA分析细胞产生的PGE(2)和白细胞介素-6。虽然龈上生物膜未引起显著变化，但龈下生物膜分别使COX-2和白细胞介素-6基因表达增强8.6倍和2.9倍，使PGE(2)和白细胞介素-6产生分别增强72.5倍和1.5倍。总之，龈下生物膜是牙龈成纤维细胞中PGE(2)的有效诱导剂，为龈下生物膜与牙周炎骨吸收的关联提供了进一步的机制见解。
