Department of Skin Care and Beauty, Osan University, Osan Cosmetology Research Institute, Konkuk University, Seoul, Korea.
Exp Dermatol. 2011 Dec;20(12):998-1003. doi: 10.1111/j.1600-0625.2011.01388.x.
Centella asiatica (C. asiatica) is a pharmacological plant in South Asia. It has been demonstrated that C. asiatica extracts containing various pentacyclic triterpenes exert healing effects, especially wound healing and collagen synthesis in skin. However, there are few studies on the effect of C. asiatica extracts on stress-induced premature senescence (SIPS). To determine whether H(2) O(2) -induced senescence is affected by C. asiatica extracts, we performed senescence analysis on cultured human dermal fibroblasts (HDFs). We also analysed whole gene expression level using microarrays and showed that 39 mRNAs are differentially expressed in H(2) O(2) -induced HDFs with and without treatment with C. asiatica extracts. These genes regulate apoptosis, gene silencing, cell growth, transcription, senescence, DNA replication and the spindle checkpoint. Differential expression of FOXM1, E2F2, MCM2, GDF15 and BHLHB2 was confirmed using semi-quantitative PCR. In addition, C. asiatica extracts rescued the H(2) O(2) -induced repression of replication in HDFs. Therefore, the findings presented here suggest that C. asiatica extracts might regulate SIPS by preventing repression of DNA replication and mitosis-related gene expression.
积雪草（C. asiatica）是南亚的一种具有药理学作用的植物。已经证实，含有各种五环三萜的积雪草提取物具有愈合作用，特别是对皮肤的愈合和胶原合成。然而，关于积雪草提取物对应激诱导的早衰（SIPS）的影响的研究较少。为了确定 H（2）O（2）诱导的衰老是否受积雪草提取物的影响，我们对培养的人真皮成纤维细胞（HDFs）进行了衰老分析。我们还使用微阵列分析了全基因表达水平，并显示 H（2）O（2）诱导的 HDFs 在有无积雪草提取物处理时，有 39 个 mRNA 表达水平存在差异。这些基因调节凋亡、基因沉默、细胞生长、转录、衰老、DNA 复制和纺锤体检查点。使用半定量 PCR 证实了 FOXM1、E2F2、MCM2、GDF15 和 BHLHB2 的差异表达。此外，积雪草提取物挽救了 H（2）O（2）诱导的 HDFs 中复制的抑制。因此，本研究结果表明，积雪草提取物可能通过防止 DNA 复制和有丝分裂相关基因表达的抑制来调节 SIPS。
