Chaushu Liat, Weinreb Miron, Beitlitum Ilan, Moses Ofer, Nemcovsky Carlos E
Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
J Clin Periodontol. 2015 Mar;42(3):288-93. doi: 10.1111/jcpe.12372. Epub 2015 Mar 9.
This study evaluated the effects of a topical herbal patch (PerioPatch®) for gingival wound healing in a rat model.
A mid-crestal incision was performed on each side of the edentulous anterior maxilla in 48, 6-month-old, Wistar rats. Full-thickness flaps were raised, repositioned and sutured. Four experimental groups were established: herbal patch, placebo patch, no patch and no patch and no surgery. Patches were placed immediately after surgery and replaced every 12 h for the following 3 days. Half of the animals were killed after 5 and the remaining ones after 12 days. Tissue blocks were retrieved and processed for histological and immunohistochemical evaluation. Epithelial gap, collagen contents, amount of macrophages, cellular proliferation and vascular contents were evaluated in the central incision area. Statistical analysis consisted of two-way anova.
The herbal patch group presented the smallest epithelial gap at 12 days, the highest collagen content both at 5 and 12 days, a larger number of proliferating cells at day 5 and more numerous blood vessels at day 12. Macrophage number was similar in all groups.
Herbal patch improved wound healing in this animal model.
本研究在大鼠模型中评估了一种局部用草药贴片（PerioPatch®）对牙龈伤口愈合的影响。
对48只6月龄的Wistar大鼠无牙的上颌前部两侧进行龈缘正中切口。掀起全厚瓣，复位并缝合。设立四个实验组：草药贴片组、安慰剂贴片组、无贴片组以及无贴片且未手术组。术后立即放置贴片，并在接下来的3天内每12小时更换一次。一半动物在术后5天处死，其余动物在术后12天处死。取出组织块并进行组织学和免疫组织化学评估。在中央切口区域评估上皮间隙、胶原含量、巨噬细胞数量、细胞增殖和血管含量。统计分析采用双向方差分析。
草药贴片组在术后12天时上皮间隙最小，在术后5天和12天时胶原含量最高，在术后5天时增殖细胞数量较多，在术后12天时血管数量较多。所有组中的巨噬细胞数量相似。
在该动物模型中，草药贴片促进了伤口愈合。
