Lilliehöök Inger, Tvedten Harold W
Clinical Pathology Laboratory, University Animal Hospital.
Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Vet Clin Pathol. 2011 Dec;40(4):450-458. doi: 10.1111/j.1939-165X.2011.00353.x. Epub 2011 Oct 24.
Most automated hematology analyzers cannot detect canine or feline basophils. However, many veterinary laboratories continue to report basophils as part of the automated 5-part differential leukocyte count for dogs and cats.
The study objectives were to evaluate the performance of the Sysmex XT-2000iV, Advia 2120, and CELL-DYN 3500 hematology analyzers in detecting basophils using blood from dogs, cats, and rabbits with basophilia and to investigate the concurrence of basophilia and other hematologic changes, sex, and breed in dogs.
One or more of the 3 hematology analyzers was used to analyze 11 canine blood samples with prominent basophilia (≥ 5%) based on a manual differential count. In addition, samples from 2 cats and 4 rabbits with basophilia were analyzed with the Advia 2120. Leukocyte cytograms were inspected for the likely location of basophil cell clusters. In a retrospective study of canine patients, reports of hematologic results that included a manual leukocyte differential count were identified using the laboratory information system and examined for the occurrence of basophilia and other hematologic changes, sex, and breed of the dogs.
Canine basophils were not detected by the Sysmex XT-2000iV or CELL-DYN 3500 analyzers, and neither canine nor feline basophils were detected by the Advia 2120. The Advia was able to detect basophils in rabbits. On the Sysmex cytogram canine basophils were found slightly above or together with neutrophils. On the Advia Perox cytogram canine basophils were located in upper part of the lymphocyte box and in the area of large unstained cells (LUC). Dogs with marked basophilia often had concurrent eosinophilia, and basophilia may be found more frequently in Rottweiler dogs than in other breeds. In 5 dogs with marked basophilia and without eosinophilia, marked thrombocytosis and anemia were noted.
Canine basophils were not detected by these automated hematology analyzers, and careful analysis of instrument graphical displays or increased LUC (Advia) may guide the need to examine a blood smear for basophils.
大多数自动血液分析仪无法检测犬猫嗜碱性粒细胞。然而，许多兽医实验室仍将嗜碱性粒细胞作为犬猫自动五分类白细胞计数的一部分进行报告。
本研究的目的是评估Sysmex XT - 2000iV、Advia 2120和CELL - DYN 3500血液分析仪使用患有嗜碱性粒细胞增多症的犬、猫和兔的血液检测嗜碱性粒细胞的性能，并调查犬类嗜碱性粒细胞增多症与其他血液学变化、性别和品种之间的相关性。
使用3台血液分析仪中的1台或多台，基于手工分类计数对11份嗜碱性粒细胞显著增多（≥5%）的犬血样本进行分析。此外，使用Advia 2120对2只患有嗜碱性粒细胞增多症的猫和4只患有嗜碱性粒细胞增多症的兔的样本进行分析。检查白细胞细胞图以确定嗜碱性粒细胞簇的可能位置。在一项对犬类患者的回顾性研究中，利用实验室信息系统识别包含手工白细胞分类计数的血液学结果报告，并检查犬类嗜碱性粒细胞增多症及其他血液学变化、性别和品种的发生情况。
Sysmex XT - 2000iV或CELL - DYN 3岁分析仪未检测到犬嗜碱性粒细胞，Advia 2120既未检测到犬嗜碱性粒细胞也未检测到猫嗜碱性粒细胞。Advia能够检测到兔的嗜碱性粒细胞。在Sysmex细胞图上，犬嗜碱性粒细胞位于略高于中性粒细胞或与中性粒细胞重叠的位置。在Advia Perox细胞图上，犬嗜碱性粒细胞位于淋巴细胞区域的上部和大未染色细胞（LUC）区域。患有明显嗜碱性粒细胞增多症的犬通常同时患有嗜酸性粒细胞增多症，并且罗威纳犬中嗜碱性粒细胞增多症的发生率可能高于其他品种。在5只患有明显嗜碱性粒细胞增多症且无嗜酸性粒细胞增多症的犬中，发现有明显的血小板增多症和贫血。
这些自动血液分析仪未检测到犬嗜碱性粒细胞，仔细分析仪器图形显示或增加LUC（Advia）可能有助于指导是否需要检查血涂片以查找嗜碱性粒细胞。
