Martínez-Caro Javier, Pastor Josep
Servei d'Hematologia Clínica Veterinària, Departament de Medicina i Cirurgia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.
Animals (Basel). 2024 Nov 22;14(23):3362. doi: 10.3390/ani14233362.
The Sysmex XN-1000V provides a percentage and concentration of basophils from the WNR scattergram, as for human samples, but this method has been shown to be irrelevant in cats. This study aimed to characterize the feline basophil distribution on the WDF channel and to preliminarily evaluate the performance of a new basophil gate on the WDF channel. Cases of feline basophilia were retrospectively retrieved and the scattergram from the WDF and WNR channels were evaluated for any consistent pattern. A new gating setting for the WDF channel was created to include the suspected basophil region. This new gating was applied retrospectively to identified basophilia cases and prospectively to randomly selected feline cases. Manual, WNR, and new WDF methods for basophil identification were compared. Nine cases of feline basophilia were identified. A characteristic WDF scattergram was identified in seven of the nine cases. A new gate was created on the WDF channel and applied to these and 34 additional cases. The comparison study showed that the new method of basophil quantification using the WDF scattergram correlated better with the manual method than the Sysmex XN-1000V method using the WNR scattergram. Basophil concentration in feline peripheral blood can be determined using a new gate on the WDF channel of the Sysmex XN-1000V, which provides better performance than the WNR channel and is comparable to the manual method.
Sysmex XN - 1000V如同对人类样本一样，可从WNR散点图中得出嗜碱性粒细胞的百分比和浓度，但已证明该方法对猫不适用。本研究旨在描述猫嗜碱性粒细胞在WDF通道上的分布特征，并初步评估WDF通道上新的嗜碱性粒细胞设门的性能。回顾性检索猫嗜碱性粒细胞增多症病例，并评估WDF和WNR通道的散点图是否有一致的模式。为包含疑似嗜碱性粒细胞区域创建了WDF通道的新设门设置。将此新设门回顾性应用于已确定的嗜碱性粒细胞增多症病例，并前瞻性应用于随机选择的猫病例。比较了手动、WNR和新的WDF方法对嗜碱性粒细胞的识别。共识别出9例猫嗜碱性粒细胞增多症病例。9例中有7例识别出特征性的WDF散点图。在WDF通道上创建了一个新门并应用于这些病例及另外34例病例。比较研究表明，使用WDF散点图进行嗜碱性粒细胞定量的新方法与手动方法的相关性优于使用WNR散点图的Sysmex XN - 1000V方法。使用Sysmex XN - 1000V的WDF通道上的新门可测定猫外周血中的嗜碱性粒细胞浓度，其性能优于WNR通道，且与手动方法相当。
