BioElectroMed Corp., Burlingame, California 94010, USA.
Wound Repair Regen. 2011 Sep-Oct;19(5):645-55. doi: 10.1111/j.1524-475X.2011.00723.x.
Due to the transepidermal potential of 15-50 mV, inside positive, an injury current is driven out of all human skin wounds. The flow of this current generates a lateral electric field within the epidermis that is more negative at the wound edge than at regions more lateral from the wound edge. Electric fields in this region could be as large as 40 mV/mm, and electric fields of this magnitude have been shown to stimulate human keratinocyte migration toward the wounded region. After flowing out of the wound, the current returns through the space between the epidermis and stratum corneum, generating a lateral field above the epidermis in the opposite direction. Here, we report the results from the first clinical trial designed to measure this lateral electric field adjacent to human skin wounds noninvasively. Using a new instrument, the Dermacorder®, we found that the mean lateral electric field in the space between the epidermis and stratum corneum adjacent to a lancet wound in 18-25-year-olds is 107-148 mV/mm, 48% larger on average than that in 65-80-year-olds. We also conducted extensive measurements of the lateral electric field adjacent to mouse wounds as they healed and compared this field with histological sections through the wound to determine the correlation between the electric field and the rate of epithelial wound closure. Immediately after wounding, the average lateral electric field was 122 ± 9 mV/mm. When the wound is filled in with a thick, disorganized epidermal layer, the mean field falls to 79 ± 4 mV/mm. Once this epidermis forms a compact structure with only three cell layers, the mean field is 59 ± 5 mV/mm. Thus, the peak-to-peak spatial variation in surface potential is largest in fresh wounds and slowly declines as the wound closes. The rate of wound healing is slightly greater when wounds are kept moist as expected, but we could find no correlation between the amplitude of the electric field and the rate of wound healing.
由于 15-50mV 的经皮电位，内部为正，所有人类皮肤伤口都会被逐出损伤电流。该电流的流动会在表皮内产生一个侧向电场，在伤口边缘处比在伤口边缘更外侧的区域更负。该区域的电场可能高达 40mV/mm，并且已经证明这种幅度的电场会刺激人角质形成细胞向受伤区域迁移。流出伤口后，电流通过表皮和角质层之间的空间返回，在表皮上方产生与相反方向的侧向场。在这里，我们报告了首次旨在非侵入性地测量人类皮肤伤口附近这种侧向电场的临床试验结果。使用一种新仪器，Dermacorder®，我们发现，在 18-25 岁的人中，在表皮和角质层之间的空间中，紧邻柳叶刀伤口的侧向电场的平均值为 107-148mV/mm，平均比 65-80 岁的人高 48%。我们还对小鼠伤口愈合过程中相邻的侧向电场进行了广泛的测量，并将该场与通过伤口的组织学切片进行了比较，以确定电场与上皮伤口闭合率之间的相关性。受伤后立即，平均侧向电场为 122±9mV/mm。当伤口被厚厚的、无序的表皮层填满时，平均场降至 79±4mV/mm。一旦表皮形成只有三层细胞的紧密结构，平均场为 59±5mV/mm。因此，表面电位的峰峰值空间变化在新鲜伤口中最大，并随着伤口闭合而缓慢下降。如预期的那样，当伤口保持湿润时，伤口愈合的速度会稍快一些，但我们无法发现电场幅度与伤口愈合速度之间的相关性。
