Spence D W, Pomeranz B
Department of Zoology, University of Toronto, Ontario, Canada.
Physiol Meas. 1996 May;17(2):57-69. doi: 10.1088/0967-3334/17/2/001.
We describe a new transcutaneous electrical resistance method for measuring surgical wound healing which permits repeated measurements in vivo throughout the healing period. A saline-filled silver electrode was used to monitor the increasing ohmic resistance which accompanied the healing. Two validation studies were carried out. In the first study, histology on 16 rats showed that an intact stratum corneum was responsible for the high electrical resistance of skin, since lesions of the corneum markedly lowered the resistance. In the second study, electrical resistance readings of incisional wounds were taken repeatedly over 20 days in 13 rats: regression analysis showed that the healing slope for each incisional wound had a correlation coefficient r-value greater than 0.85. Moreover, all slopes were positive (p < 0.001) demonstrating that resistance increases as wound healing progresses and hence is a valid measure of healing. Test-retest resistance readings showed high reliability when measurements were repeated on the same wound (Pearson r = 0.94, n = 110). Hence we have shown that this new method is both valid and reliable. The device only monitors epidermal repair and gives no indication of wound strength. A modified device recently developed for humans has been used successfully on post-surgical wounds in numerous patients.
我们描述了一种用于测量手术伤口愈合情况的新型经皮电阻测量方法，该方法能够在整个愈合期内对活体进行重复测量。使用一个充满盐水的银电极来监测随着愈合过程而增加的欧姆电阻。进行了两项验证研究。在第一项研究中，对16只大鼠进行的组织学检查表明，完整的角质层是皮肤高电阻的原因，因为角质层受损会显著降低电阻。在第二项研究中，对13只大鼠的切口伤口在20天内反复进行电阻读数测量：回归分析表明，每个切口伤口的愈合斜率的相关系数r值大于0.85。此外，所有斜率均为正值（p < 0.001），表明随着伤口愈合进展电阻增加，因此是愈合情况的有效测量指标。当在同一伤口上重复测量时，重测电阻读数显示出高可靠性（Pearson r = 0.94，n = 110）。因此，我们已经证明这种新方法既有效又可靠。该装置仅监测表皮修复情况，无法显示伤口强度。最近为人类开发的一种改良装置已成功应用于众多患者的术后伤口。
