Rose Lara J, Dunn Marilyn E, Allegret Virginie, Bédard Christian
Department of Clinical Sciences, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec, Canada.
Charles River Laboratories, Senneville, Québec, Canada.
Vet Clin Pathol. 2011 Dec;40(4):426-434. doi: 10.1111/j.1939-165X.2011.00364.x. Epub 2011 Nov 3.
Long-term corticosteroid therapy has been associated with increased risk of thrombotic disease in dogs.
The purpose of this prospective study was to use thrombelastography (TEG) and thrombin generation (TG) to detect development of a hypercoagulable state in healthy Beagle dogs receiving oral prednisone. We hypothesized that administration of corticosteroids would result in a hypercoagulable profile on TEG tracings and an increase in TG.
Six healthy adult Beagles from the University of Montreal's research colony were used to conduct a prospective longitudinal study in which all dogs received 1 mg/kg of prednisone orally once daily for 2 weeks, followed by a 6-week washout period, and then 4 mg/kg of prednisone orally once daily for 2 weeks. TEG tracings on citrated whole blood and TG measurements on frozen-thawed platelet-poor plasma were obtained before prednisone administration (baseline), at the end of the washout period, and at the end of both corticosteroid trials.
Significant differences compared with baseline values were obtained for K, α, and MA, with tracings compatible with a hypercoagulable profile following both corticosteroid trials. There was a significant increase in endogenous thrombin potential only after low-dose (1 mg/kg) prednisone.
Administration of prednisone to healthy Beagles resulted in hypercoagulability as indicated by TEG tracings, whereas the effect on TG was more variable. Further studies are needed to determine the underlying mechanisms of hypercoagulability and its clinical impact.
长期使用皮质类固醇治疗与犬类血栓性疾病风险增加有关。
这项前瞻性研究的目的是使用血栓弹力图（TEG）和凝血酶生成（TG）来检测接受口服泼尼松的健康比格犬高凝状态的发展。我们假设给予皮质类固醇会导致TEG描记图呈现高凝状态，并使TG增加。
来自蒙特利尔大学研究群体的6只健康成年比格犬用于进行一项前瞻性纵向研究，所有犬只每天口服1mg/kg泼尼松，持续2周，随后有6周的洗脱期，然后每天口服4mg/kg泼尼松，持续2周。在给予泼尼松前（基线）、洗脱期末以及两个皮质类固醇试验结束时，获取枸橼酸化全血的TEG描记图以及冻融后少血小板血浆的TG测量值。
与基线值相比，K、α和MA有显著差异，两个皮质类固醇试验后的描记图均符合高凝状态。仅在低剂量（1mg/kg）泼尼松后内源性凝血酶潜力有显著增加。
对健康比格犬给予泼尼松会导致TEG描记图显示高凝状态，而对TG的影响则更具变异性。需要进一步研究以确定高凝状态的潜在机制及其临床影响。
