Department of Chemistry, Sensor and Biosensor Research Center (SBRC) & Nanoscience and Nanotechnology Research Center (NNRC), Faculty of Science, Razi University, P.O. Box: 67149, Kermanshah, Iran. kashanian
Spectrochim Acta A Mol Biomol Spectrosc. 2012 Feb;86:351-9. doi: 10.1016/j.saa.2011.10.048. Epub 2011 Oct 25.
The interaction of native calf thymus DNA (CT-DNA) with [Cu(ph(2)phen)(phen-dione)Cl]Cl was studied at physiological pH by spectrophotometric, spectrofluorometric, circular dichroism, and viscometric techniques. Considerable hypochromicity and red shift are observed in the UV absorption band of the Cu complex. Binding constants (K(b)) of DNA with the complex were calculated at different temperatures. Thermodynamic parameters, enthalpy and entropy changes were calculated according to Van't Hoff equation, which indicated that reaction is predominantly enthalpically driven. All these results indicate that Cu(II) complex interacts with CT-DNA via intercalative mode. Also, this new complex induced cleavage in pUC18 plasmid DNA as indicated in gel electrophoresis and showed excellent antitumor activity against K562 (human chronic myeloid leukemia) and human T lymphocyte carcinoma-Jurkat cell lines.
采用分光光度法、荧光光谱法、圆二色光谱法和黏度法研究了生理 pH 值下[Cu(ph(2)phen)(phen-dione)Cl]Cl 与天然小牛胸腺 DNA（CT-DNA）的相互作用。Cu 配合物的紫外吸收带观察到明显的减色和红移。在不同温度下计算了 DNA 与配合物的结合常数（K(b)）。根据范特霍夫方程计算了热力学参数、焓变和熵变，表明反应主要是由焓驱动的。所有这些结果表明，Cu(II)配合物通过嵌入模式与 CT-DNA 相互作用。此外，该新型配合物在凝胶电泳中可诱导 pUC18 质粒 DNA 断裂，并对 K562（人慢性髓系白血病）和人 T 淋巴细胞白血病-Jurkat 细胞系表现出优异的抗肿瘤活性。
