Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, Department of Chemistry, Hubei Normal University, Huangshi, 435002, People's Republic of China.
Mol Biol Rep. 2013 Jul;40(7):4405-13. doi: 10.1007/s11033-013-2530-6. Epub 2013 May 5.
Curcumin is a polyphenolic bioactive compound found in the spice turmeric endowed with diverse pharmacological and biological activities. In this study, fluorescence spectroscopy in combination with UV-Vis absorbance spectroscopy was employed to investigate the high affinity binding of curcumin to herring sperm DNA (hs-DNA). From the series of studies undertaken in the present program, for example, steady-state emission; absorption; the effect of denaturants; competition experiment; and anion (iodide) ion-induced fluorescence quenching; the mode of binding of curcumin into the DNA helix has been substantiated to be principally intercalative. Binding parameters calculating from Stern-Volmer method and Scatchard method showed that curcumin bind to hs-DNA with the binding affinities of the order 10(4 )L mol(-1). The effects of ionic strength, chemical denaturants, thermal denaturation and pH were studied to show the factors of the interaction, and provided further support for the intercalative binding mode. In addition, the methods and techniques used in the present work can also be exploited to study the interaction of curcumin with other biological, biomimicking assemblies and drug delivery vehicles, and so forth.
姜黄素是一种存在于香料姜黄中的多酚类生物活性化合物，具有多种药理学和生物学活性。在这项研究中，荧光光谱学结合紫外可见吸收光谱学被用来研究姜黄素与鲱鱼精子 DNA（hs-DNA）的高亲和力结合。从本研究计划进行的一系列研究中，例如，稳态发射；吸收；变性剂的影响；竞争实验；和阴离子（碘化物）离子诱导的荧光猝灭；姜黄素进入 DNA 螺旋的结合模式已被证实主要是嵌入的。从 Stern-Volmer 法和 Scatchard 法计算的结合参数表明，姜黄素与 hs-DNA 具有 10(4)L mol(-1)数量级的结合亲和力。研究了离子强度、化学变性剂、热变性和 pH 值的影响，以显示相互作用的因素，并为嵌入结合模式提供了进一步的支持。此外，本工作中使用的方法和技术也可用于研究姜黄素与其他生物、仿生组装体和药物传递载体等的相互作用。
