Ren Ting, Wang Zechun, Zhang Lijiao, Wang Ning, Han Xinxin, Wang Ruiyong, Chang Junbiao
College of Chemistry and Molecular Engineering, Zhengzhou University, 100 Science Avenue, Zhengzhou, 450001, China.
J Fluoresc. 2017 Jul;27(4):1467-1477. doi: 10.1007/s10895-017-2086-2. Epub 2017 Apr 11.
In this work, the interaction between camptothecin (CPT) analogs and fat mass and obesity associated (FTO) was investigated using spectroscopy and molecular docking. From the experimental results, it was found that the CPT analogs caused the fluorescence quenching of FTO through a static quenching procedure. The binding constants and thermodynamic parameters at three different temperatures, the number of binding sites were obtained, which suggested that the hydrophobic interaction and electrostatic force played major role in the reaction between CPT analogs and FTO. Results revealed that 10-hydroxycamptothecin was the strongest quencher.
在这项工作中，使用光谱学和分子对接研究了喜树碱（CPT）类似物与脂肪量和肥胖相关蛋白（FTO）之间的相互作用。从实验结果发现，CPT类似物通过静态猝灭过程导致FTO的荧光猝灭。获得了三个不同温度下的结合常数和热力学参数、结合位点数量，这表明疏水相互作用和静电力在CPT类似物与FTO的反应中起主要作用。结果显示10-羟基喜树碱是最强的猝灭剂。
