Laboratório Nacional de Energia e Geologia (LNEG), Unidade de Bioenergia, Estrada do Paço do Lumiar 1649-038 Lisboa, Portugal.
Bioresour Technol. 2012 Jan;104:342-8. doi: 10.1016/j.biortech.2011.10.059. Epub 2011 Oct 25.
The present work deals with the optimization of cellular disruption and sugar extraction from the microalgae Scenedesmus obliquus (Sc) for bioethanol production. Among the physical and physicochemical methods tested, the best results were obtained with acid hydrolysis by H(2)SO(4) (2N), at 120 °C for 30 min and using dried biomass. The sugar extraction efficiency level reached was 95.6% when compared to the harsh quantitative acid hydrolysis. The influence of other parameters such as biomass loading and number of extraction cycles were also evaluated. The results obtained in the latter case showed that a unique hydrolysis step is sufficient.
本工作研究了从微藻斜生栅藻（Sc）中细胞破碎和糖提取以用于生物乙醇生产的优化。在所测试的物理和物理化学方法中，用 2N H(2)SO(4)在 120°C 下加热 30 分钟对干燥生物质进行酸水解得到了最佳结果。与苛刻的定量酸水解相比，糖提取效率水平达到了 95.6%。还评估了其他参数（如生物质负荷和提取循环次数）的影响。在后一种情况下获得的结果表明，仅需一个水解步骤即可。
