Assunção Joana, Batista Ana Paula, Manoel João, da Silva Teresa Lopes, Marques Paula, Reis Alberto, Gouveia Luísa
LNEG-National Laboratory of Energy and Geology-Bioenergy Unit Lisbon Portugal.
LEAF-Linking Landscape Environment, Agriculture and Food, Instituto Superior de Agronomia Lisboa Portugal.
Eng Life Sci. 2017 Aug 22;17(10):1126-1135. doi: 10.1002/elsc.201700075. eCollection 2017 Oct.
The atmospheric CO increase is considered the main cause of global warming. Microalgae are photosynthetic microorganisms that can help in CO mitigation and at the same time produce value-added compounds. In this study, , , and were cultivated under 0.035 (air), 5 and 10% (v/v) of CO concentrations in air to evaluate the performance of the microalgae in terms of kinetic growth parameters, theoretical CO biofixation rate, and biomass composition. Among the microalgae studied, presented the highest values of specific growth rate ( = 1.28 d), maximum productivities ( = 0.28 g Ld), and theoretical CO biofixation rates (0.56 g Ld) at 10% CO. The highest oil content was found at 5% CO, and the fatty acid profile was not influenced by the concentration of CO in the inflow gas mixture and was in compliance with EN 14214, being suitable for biodiesel purposes. The impact of the CO on cells' viability/cell membrane integrity evaluated by the flow cytometry is quite innovative and fast, and revealed that 86.4% of the cells were damaged/permeabilized in cultures without the addition of CO.
大气中二氧化碳的增加被认为是全球变暖的主要原因。微藻是光合微生物，有助于减少二氧化碳排放，同时还能产生增值化合物。在本研究中，[此处原文缺失具体微藻种类名称]在空气中二氧化碳浓度分别为0.035%（空气）、5%和10%（v/v）的条件下进行培养，以评估微藻在动力学生长参数、理论二氧化碳生物固定率和生物质组成方面的性能。在所研究的微藻中，[此处原文缺失具体微藻种类名称]在10%二氧化碳浓度下呈现出最高的比生长速率（μ = 1.28 d⁻¹）、最大生产力（Pmax = 0.28 g L⁻¹ d⁻¹）和理论二氧化碳生物固定率（0.56 g L⁻¹ d⁻¹）。在5%二氧化碳浓度下发现了最高的油含量，并且脂肪酸谱不受流入气体混合物中二氧化碳浓度的影响，符合EN 14214标准，适用于生物柴油用途。通过流式细胞术评估二氧化碳对[此处原文缺失具体微藻种类名称]细胞活力/细胞膜完整性的影响具有创新性且快速，结果显示在不添加二氧化碳的培养物中，86.4%的细胞受损/通透。
