Tajimaya Co. Ltd., 717 Kamitakatsu, Tsuchiura, Ibaraki 300-0811, Japan.
Bioresour Technol. 2012 Jan;104:791-4. doi: 10.1016/j.biortech.2011.10.017. Epub 2011 Oct 28.
Effects of Ca(OH)(2), NH(4)OH, and NaOH as neutralizing agents for efficient recovery of lactic acid was investigated. Lactic acid was produced from broken rice in a simultaneous saccharification and fermentation (SSF) process with Lactobacillus delbrueckii. Consumption of glucose (from broken rice) by the cells and the cell growth were the best with Ca(OH)(2) among the three neutralizing agents used. Maximum productivities of lactic acid reached with Ca(OH)(2), NH(4)OH, and NaOH were 3.59 g l(-1) h(-1), 1.51 g 1(-1) h(-1), and 1.40 g l(-1) h(-1), respectively. Ca(OH)(2) reduced the lactate molarity of the fermentation broth, and thus resulted in the highest lactic acid productivity. Furthermore, it was apparently clear that divalent cation (Ca(2+)) was more effective in neutralizing the cultures compared to monovalent (Na(+) and NH(3)(+)) cations.
研究了 Ca(OH)(2)、NH(4)OH 和 NaOH 作为中和剂对高效回收乳酸的影响。乳酸是由乳酸乳杆菌在破碎米的同步糖化发酵（SSF）过程中产生的。在三种中和剂中，Ca(OH)(2) 最有利于细胞消耗葡萄糖（来自破碎米）和细胞生长。达到最大乳酸产率的 Ca(OH)(2)、NH(4)OH 和 NaOH 的产率分别为 3.59 g l(-1) h(-1)、1.51 g 1(-1) h(-1) 和 1.40 g l(-1) h(-1)。Ca(OH)(2) 降低了发酵液中的乳酸摩尔浓度，从而导致最高的乳酸产率。此外，很明显二价阳离子（Ca(2+))比一价（Na(+)和 NH(3)(+))阳离子更有效地中和培养物。
